临床医学论文-葛根素对低O2高CO2肺动脉高压大鼠肺动脉平滑肌细胞线粒体途径凋亡的影响.doc

《临床医学论文-葛根素对低O2高CO2肺动脉高压大鼠肺动脉平滑肌细胞线粒体途径凋亡的影响.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、=1临床医学论文■葛根素对低02高C02肺动脉高压大鼠肺动脉平滑肌细胞线粒体途径凋亡的影响【摘要】H的：研究在慢性低02高C02情况下，葛根素对肺动脉平滑肌细胞(PASMCs)线粒体途径凋亡的影响。方法:雄性SD大鼠40只，随机分为正常对照组(N组)、慢性低02高C022周组(H2w组)、慢性低02高C024周组(H4w组)、慢性低02高C024周+葛根素组(H4w+G组)，每组10只。TUNEL法检测PASMCs凋亡；免疫组化测定PASMCs线粒体细胞色索C(Cyt-c)释放量及胞浆Bcl・2、Ba

2、x含量；分光光度法检测肺组织匀浆caspase9酶活性。结果:①H2w组、H4w组和H4w+G组mPAP、RV/(LV+S)>PAMT、PASMCs胞浆Bcl-2含量均显着高于N组(P<0.01),Cyt-c释放量、Bnx含量及帅组织匀浆caspase9酶活性均显着低于N组(P<0.01)；H2w组、H4w组各级PASMCs和H4w+G组肺弹力型动脉和肺微细动脉PASMCs凋亡指数显着低于N组(P<0.01)o②H4w+G组mPAP、RV/(LV+S)>PAMT、肺弹力型动脉和肺微细动脉PASMCs屮

3、Bcl・2含量均显着低于H4w组(P<0.01)；弹力型动脉和肌型动脉PASMCs凋亡指数、Cyt-c释放量,帅组织匀浆caspase9酶活性均显着高于H4w组(P<0.01)；Bax含］量在H4w+G组与H4w组间差异无显着性(P>0.05)o结论:葛根素有抑制慢性低02高CO2肺动脉高压和肺血管重建的作用，促进PASMCs通过线粒体途径凋亡可能是其作用机制之一。【关键词】低氧慢性高血压肺性线粒体凋Abstract:Objective:Tostudytheeffectsofpuerarinonmit

4、ochondria1・dependentapoptosispathwayinpulomnaryhypertensioninducedbyhypoxichypercapnia.Methods:FourtySprague-Dawleyratswererandomlydividedintofourgroups:normalcontrolgroup(N).hypoxichypercapnic2weeksgroup(H2w),hypoxichypercapnic4weeksgroup(H4w)andhypoxi

5、chypercapnic4weeks+Pucraringroup(H4w-K3)・TheapoptsisofPASMCswastestedwithTUNEL.ThereleaseofCyl-cfrommitochondriaandthecontentofBcl・2andBaxincytoplasmweredeterminedwithimmunohistochemistry.Theactivityofcaspase9wasdetectedwithspectropho-lometricmethod.Res

6、ults:①ThemPAP,RV/(LV+S),PAMTandthecontentofBcl・2ofPASMCsofgroupH2w,groupH4wandgroupH4w+GweresignificantlyhigherthanthoseofgroupN(P<0.01)・ThereleaseofCyt・c,thecontentofBaxofPASMCsandtheactivityofcaspase9oflungingroupH2w,groupH4wandgroupH4w+Gweresignifica

7、ntly1owerthanthoseingroupN(P<0.01)•TheapoplolicindexofPASMCsingroupH2w,groupH4wandpulmonaryclasticarteryandpulmonaryartcrolcsofgroupH4w+Gweresignificantly1owerthanthoseingroupN(P<0.01)・②ThemPAP.RV/(LV+S),PAMTandthecontentofBcl・2inpulmonaryclasticarterya

8、ndartcrolcsofgroupH4w+Gweresignificantly1owerthanthoseofgroupH4w(P<0.01)・ThereleaseofCyl・c,theapoptolicindexofPASMCsinpulmonaryelasticarteryandpulmonarymusculararteryandtheactivityofcaspase9oflungingroupH4w+Gweresignificantlyhigh