TLR4在晚发型新生儿败血症中的意义.doc

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1、［摘要］目的探讨Toll样受体4(TLR4)对晚发型新生儿败血症诊断的临床意义。方法选取2011年8月〜2014年11月我院收治的血培养阳性的新生儿38例作为败血症组，根据感染病原体将其分为革兰阳性菌感染组(G+组)23例，革兰阴性菌感染组(G-组)15例，另选取我院同期收治的无感染性疾病的新生儿20例作为对照组，采集各组患儿外周血，应用密度梯度离心法分离出外周血单个核细胞，采用RT-PCR法检测TLR4mRNA表达水平,免疫投射比浊法检测血清CRP水平。比较组间各指标的差异，并分析两者的相关性。

2、结果G+组与G-组TLR4mRNA及CRP水平与对照组相比均明显升高(P0.05)；G-组TLR4mRNA的表达明显咼于G+组(P［关键词］Toll样受体4；C反应蛋白；新生儿；败血症［中图分类号］R722.1［文献标识码］A［文章编号］1673-9701(2016)12-0011-03SignificanceofTLR4inthelate-onsetneonatalsepticemiaZHOUQinlZHANGQinlZHOUSuya21.DepartmentofPediatrics,Zheji

3、angPeople'sHospital,Hangzhou310014,China；2.DepartmentofNeonatology，HangzhouChildren'sHospital,Hangzhou310006、China[Abstract]ObjectiveToinvestigatetheclinicalsignificanceoftoll-likereceptor4(TLR4)inthediagnosisoflate-onsetneonatalsepticemia・MethodsAtot

4、alof38newbornswithpositivebloodculturewhowereadmittedfromAugust2011toNovember2014wereselectedasthesepticemiagroup.Accordingtotheinfectionpathogens,thenewbornsweredividedintogrampositivebacteriainfectiongroup(G+)with23cases，gramnegativebacteriainfectio

5、ngroup(G-)with15casesand20newbornswithoutinfectiousdiseasesfromthesameperiodoftimewereselectedasthecontrolgroup・Theperipheralbloodofthenewbornswascollectedandperipheralbloodmononuclearcellswereisolatedwiththedensitygradientcentrifugation.Theexpression

6、levelofTLR4mRNAwasdetectedwithRT-PCRmethodandserumCRPlevelwasdetectedwithimmunitytransmissionturbidity.Comparethedifferencesofeachindexofthetwogroupsandanalyze1.DepartmentofPediatrics,ZhejiangPeople'sHospital,Hangzhou310014,China；2.DepartmentofNeonato

7、logy，HangzhouChildren'sHospital,Hangzhou310006、China[Abstract]ObjectiveToinvestigatetheclinicalsignificanceoftoll-likereceptor4(TLR4)inthediagnosisoflate-onsetneonatalsepticemia・MethodsAtotalof38newbornswithpositivebloodculturewhowereadmittedfromAugus

8、t2011toNovember2014wereselectedasthesepticemiagroup.Accordingtotheinfectionpathogens,thenewbornsweredividedintogrampositivebacteriainfectiongroup(G+)with23cases，gramnegativebacteriainfectiongroup(G-)with15casesand20newbornswithoutinfectiousdis