冠状病毒Coronavirusl论文-2012 Alphacoronavirus Detected in Bats in the United Kingdom.pdf

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1、VECTOR-BORNEANDZOONOTICDISEASESVolume12,Number6,2012ªMaryAnnLiebert,Inc.DOI:10.1089/vbz.2011.0829AlphacoronavirusDetectedinBatsintheUnitedKingdomTomA.August,FionaMathews,andMilesA.NunnAbstractThisstudypresentsthefirstrecordofcoronavirusinBritishbats.Alphacoronavirusstrainsweredetectedintwoofseven

2、batspecies,namelyMyotisnattereriandM.daubentonii.VirusprevalencewasparticularlyhighinthepreviouslyunrecognizedhostM.nattereri,whichcanliveincloseproximitytohumans.KeyWords:Chiroptera—Disease—DNAsequence—Epidemiology—Phylogeny—RT-PCR—Severeacuterespiratorysyndrome—UnitedKingdom—Virus—Zoonoses.Int

3、roductionbastellus,Myotisdaubentonii,M.nattereri,Plecotusauritus,Rhinolophusferrumequinum,andR.hipposideros.Zoonoticinfectionsoriginatinginwildlifeareasignificantthreattohumanhealth(Daszaketal.2000).MaterialsandMethodsInadditiontotheirrecognizedlinkwithrabiestransmission,batshavebeenidentifiedasth

4、ereservoirhostsofanumberSampleswerecollectedatsitesinsouthwestEnglandbe-ofvirusesthathavecauseddiseaseoutbreaksinthepasttween2006and2009.AsinglefecalpelletwascollectedfromÔdecade(Wongetal.2007).TheBetacoronavirus,severeacuteeachbat,andeitherpreservedin250lLRNAlater(Appliedrespiratorysyndrome-rel

5、atedcoronavirus(SARS-CoV),Biosystems,Warrington,Cheshire,U.K.)orsnap-frozenonwhichprobablyoriginatedininsectivorousbats(Lietal.dryice.Sampleswerestoredat-80°Cuntilanalysis.Cotton2005),causedperhapsthemostsignificantoftheseoutbreaksbagsinwhichthebatswereheldweresterilizedbetweenin2002,resultingin8

6、096humancasesand774fatalitiesusebyautoclavingfollowedbysoakingin6%sodiumacross26countries(WorldHealthOrganization,2004).hypochlorite(domesticbleach)andwashingtopreventSubsequentsurveillancedetectedcoronavirus(CoV)speciesinbatsfromeverycontinenttheyinhabit,includingconti-nentalEurope(GenBanktaxon

7、omydata,January2011),butTable1.PrevalenceofCoronavirusinSevenSpeciescoronaviruseshavenotpreviouslybeenidentifiedinbatsinofBritishBatsbyReverseTranscriptase-PolymerasetheUnitedKingdom.ChainReactionAnalysisofFecalSamplesCoronav