冠状病毒Coronavirusl论文-2005 Investigation into the causes of canine infectious respiratory disease_ antibody responses to canine respiratory co.pdf

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1、ArchVirol(2005)150:14931504DOI10.1007/s00705-005-0533-xInvestigationintothecausesofcanineinfectiousrespiratorydisease:antibodyresponsestocaninerespiratorycoronavirusandcanineherpesvirusintwokennelleddogpopulationsK.ErlesandJ.BrownlieDepartmentofPathologyandInfectiousDiseases,TheRoyalVe

2、terinaryCollege,NorthMymms,U.K.ReceivedNovember4,2004;acceptedFebruary28,2005PublishedonlineApril21,2005
cSpringer-Verlag2005Summary.Twotrainingcentresforworkingdogsweremonitoredforoneyeartodeterminethepresenceofvirusesandviralantibodiesandtheirassociationwithcanineinfectiousrespirator

3、ydisease(CIRD).Tonsillarswabsandserumwereobtainedfromdogsonentryintothekennelsandinregularintervalsthereafter.AdditionalsampleswerecollectedduringoutbreaksofCIRD.TheswabswereexaminedbyviruscultureandPCRforcanineparainfluenzavirus,canineadeno-virus,canineherpesvirus(CHV)andcaninerespirat

4、orycoronavirus(CRCoV).FurthermoretheprevalenceofantibodiestoCHVandCRCoVwasdetermined.DuringthisstudyCIRDwasreportedmainlyinoneofthetwokennelsinvestigated.InthatkennelantibodyresponsestoCRCoVindicatedaseasonaloccurrenceofthevirus,whichcoincidedwithtwooutbreaksofrespiratorydisease.CHVant

5、ibodyresponsesweredetectedthroughouttheyear.Intheotherkennel,whichreportedfewcasesofCIRDahighprevalenceofantibodiestoCRCoVwasdetectedonentrybutonlysporadicseroconversionstoCRCoVorCHV.ByPCRthreedogswerefoundpositiveforCRCoVinonekennelwhereasallPCRtestsforotherviruseswerenegativeforbothk

6、ennels.Virusculturefailedtodetectanyvirusesineitherkennel.IntroductionCanineinfectiousrespiratorydisease(CIRD)orkennelcoughisadiseasecomplex,whichoccursfrequentlyindenselyhouseddogpopulationsespeciallyafternewdogshavebeenintroduced.[1].Theproblemiswellknowninrehomingandboardingkennelsh

7、oweveritisalsoanimportantdiseaseofworkingdogsbecauseitdisruptstheirtrainingandcanevenresultintemporaryclosureof1494K.ErlesandJ.Brownliekennels.ManyfactorscontributetotheaetiologyofCIRDincludingvirusesandbacteriaaswellasstressduetomixingandhousinginanunfamiliarenvironment.Vaccinesarea