冠状病毒Coronavirusl论文-1991 [Advances in Experimental Medicine and Biology] Coronaviruses and their Diseases Volume 276 __ Background Paper Map.pdf

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1、BACKGROUNDPAPERMAPPINGEPlTOPESONCORONAVIRUSGLYCOPROTEINSHubertLaudeInstitutNationaldelaRechercheAgronomiqueStationdeRecherchesdeYirologieetd'ImmunologieMoleculaires78350Jouy-en-JosasFranceINTRODUCTIONThepurposeofthispaperistosummarisethestateofcoronavirusep

2、itoperesearch,withanemphasislaidonthemostrecentachievements,i.e.thecorrelationbetweenantigenicityandpolypeptideprimarystructure.Theantigenicpropertiesofcoronavirusesresidemainlyintheirenvelopeglycoproteins:theS(spike)protein,ahighmolecularweightpolypeptidew

3、hichconstitutesthepetal-shapedpeplomersandtheM(membrane)protein,asmallerintegralmembranepolypeptide.Somecoronavirusespossessanadditionalsurfaceglyco•protein,thehaemagglutinin-esterase(HE)protein.PEPLOMERPROTEINSAnalysisofthepredictedaminoacidsequencesnowest

4、ablishedfor5coronaviruses(IBY,MHY,TGEY,FIPYandBCY)hasledtoamodelinwhichtheproteinisdividedinto2domains:theamino-terminalhalf,whichisassumedtoformtheglobularpartofthepeplomer,andthecarboxy-terminalhalf,whichisassumedtoformanelongatedstructure,thestalkofthepe

5、plomer,andalsocontainsaputativetransmembraneanchoringregionnearthecarboxyterminus.Bothpartsbearnumerousasparagine-linkedcomplexglycansidechains,whichmaymodulatetheirantigenicity.Thesequencesoftheamino-terminalhalvesexhibitverylittleconservation,whereashomol

6、ogiesaveraging30%areobservedinthecarboxy-terminalhalves.Severalcoronaviruses,includingmY,MHYandBCYhaveaSproteinwhichiscleavedapproximatelyinthemiddle,thusdefininganamino-terminalsubunit(SI)andacarboxy-terminalsubunit(S2).Severalmajorantigenicsiteshavebeenid

7、entifiedontheSproteinsofMHY,TGEYandmybycompetitivebindingexperiments;anumberofthesesitescouldbeoriented,ifnotpreciselylocalised,ontheprimarystructureofthemolecule.MHYSProteinRecombinantvirusesgeneratedinvivobyRNA-RNArecombinationwhichcontainapproximatelythe

8、5'two-thirdsoftheMHY-JHMpeplomergeneandthe3'one-thirdoftheMHY-A59peplomergenehavelosttheirreactivitytomonoclonalantibodies(MAbs)defmingthesitesdesignatedAandBontheJHMSprotein.Itwasinferredthatbothsites