资源描述：
《冠状病毒Coronavirusl论文-1996 Growth characteristics and protein profiles of prototype and wild-type rat coronavirus isolates grown in a cloned s.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、VirusResearchELSEVIERVirusResearch41(1996)5568Growthcharacteristicsandproteinprofilesofprototypeandwild-typeratcoronavirusisolatesgrowninaclonedsublineofmousefibroblasts(L2p.176cells)DianeJ.Gaertner*,SusanR.Compton,DeborahF.Winograd,AbigailL.SmithSecti
2、onofComparativeMedicine,YaleUniversitySchoolofMedicine,P.O.Box208016,NewHaven,CT06520-8016,USAReceived14March1995;revised5December1995;accepted5December1995AbstractRatcoronaviruses(RCVs)infectlaboratoryratsandconfoundbiomedicalresearchresults.Invitrosy
3、stemsdevelopedsofarhavelimitedthegrowthinknowledgeaboutRCVsbynotpermittinggenerationofplaque-clonedvirusstocks,reliableisolationofRCVsfromrattissues,orgrowthofhightiteredstocksofallisolates.Duetothefactthatlessthan20%ofL2(Percy)cellswerebecominginfecte
4、d,sublineswereproducedandselectedformaximalgrowthofRCVs.Screeningof238cellsublinesyieldedL2p.176cellswhichwerehighlysusceptibletoallRCVstested;however,susceptibilitydeclinedafter30passagesinvitro.Low-passagedL2p.176cellsWereusedtoisolatevirusfromnatura
5、loutbreaksandtopropagateindividualRCVplaquesintohightiteredstocks.ProteinsfromsixRCVisolateswereimmunoblottedusingpolyclonalratandmouseantibodiestosialodacryoadenitisvirusandpolyclonalmonospe-cificrabbitandgoatantibodiesagainstthepeplomer(S)andnucleoca
6、psid(N)proteinsofmousehepatitisvirus(MHV).Proteinsoftwoprototype,oneJapaneseandthreewildtypeRCVswereexaminedandfoundtobesimilartothoseofMHV,althoughtheexactsizesandratiosofproteinformswereuniqueformostRCVisolates.Thisstudyreportsthedevelopmentofacontin
7、uouscelllinewhichreliablysupportsRCVsopeninganopportunityforfurtherinvivostudiesofthebiologyoftheseagents.AsafirststepinthecharacterizationofRCVs,wehaveshownthatRCVproteinsareverysimilartothoseofMHV.Keywords:Ratcoronavirus;Rat;Cellline;Cellculture;Viru
8、sstrains;SialodacryoadenitisvirusI.Introductionmaybeeithersubclinicalorclinicallyapparentandcanconfoundresearchresults(Jacoby,1986;Ratcoronaviruses(RCVs)areagentswhichBoschertetal.,1988;Huangetal.,1989;Utsumicommonlyinfectlaboratoryrats
