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1、VirusResearch,9(1988)133-144133ElsevierVRR00378GrowthpatternofvariousJHMcoronavirusisolatesinprimaryratglialcellculturescorrelateswithdifferingneurotropisminvivoPaul.T.Massa,HelmutWegeandVolkerterMeulenInstitutftirVirologieundImmunobiologiederUniuersi
2、tiitWiirzburg,Wiirzburg,F.R.G.(Acceptedforpublication24September1987)SummaryTheJHMstrainofmurinehepatitiscoronavirusisneurotropicinrats,causingeitherfatalacuteencephalomyelitisorsubacutedemyelinatingencephalomyelitis.Wehaveexaminedthegrowthpropertieso
3、fthreeJHMvirusisolatesinprimaryratglialculturesandfoundacorrelationwiththeirabilitytocausedisease.WildtypeJHMvirushasthepropensitytocauselyticinfectionsinglialcultures,andatemperature-sensitivemutantdesignatedJHM-ts43invariablyproducespersistentinfect
4、ionswithreducedcytopathiceffects(CPE)ascomparedtothewildtype.Moreover,anon-neurotropicisolate,designatedJHM-Pivirus,produceseithernon-productivepersistentinfectionsatlowmultiplicityofinfection(m.o.i.)orproductivepersistentinfectionsathighm.o.i.,with,h
5、owever,noCPE.Thepheno-typicexpressionofpersistenceisglialcell-dependent,sinceallthreevirusesproducesimilarlylyticinfectionswhengrownonvarioussusceptiblecelllines.ThegeneticbasisofJHMviruspersistencecanbeexplainedatthelevelofdirectvirus-glialcellintera
6、ctions.Murinehepatitisvirus;Braincells;Cytopathiceffects;GeneticcontrolIntroductionSeveralmurinecoronavirusstrainscauseinrodentsabroadspectrumofacutetochroniccentralnervoussystemdiseaseswhichareimportantinstudyingtheCorrespondenceto:P.T.Massa,Institut
7、fiirVirologieundImmunobiologiederUniversitltWiirzburg,VersbacherStr.7,D-8700wiirzburg,F.R.G.0168-1702/88/$03.5001988ElsevierSciencePublishersB.V.(BiomedicalDivision)134mechanismsofpersistencyanddemyelination(Sorensenetal.,1980;StohlmanandWeiner,1981;K
8、nobleretal.,1981;Sorensenetal.,1982;Lavietal.,1984).InfectionofLewisratswiththeJHMstrainofmurinecoronavirusinparticularcanleadtoademyelinatingencephalomyelitis,whichistriggeredbyapersistentvirusinfectionandisassociatedwithautoimmunereactionsag
