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2、ler,T.SchlappandW.HerbstInstitutf~rHygieneundInfektionskrankheitenclerTierederJustus-Liebig-Universit~t,Giessen,Germanyspecies.ThereareseveraldifferentmodelsforcoronavirusThenucleotidesequencesoftheregionsbetweenmRNAsynthesis.Theleader-primedtranscrip
3、tionmodelthemembraneandspikeproteingenesofthreeproposesinteractionoftheleadersequencewiththefull-lengthstrainsofporcinehaemagglutinatingencephalo-minus-strandRNAattheconsensusintergenicsequencetomyelitisvirus(HEV)weredetermined.Atotalof739initiatesynt
4、hesisofsubgenomicplus-strandRNAs.Incontrast,(HEVstrain67N)and751(strainsNT9andVW572)themodelofdiscontinuoustranscriptionduringnegative-nucleotidesweresequenced.TwoORFs,potentiallystrandRNAsynthesissuggestssubgenomicminus-strandencodingproteinsof12-8an
5、d9-6kDa,wereRNAs,containinganantileadersequenceatthe3'end,serveasidentified.Pairwisecomparisonswiththecorre-templatesfortranscriptionofplus-strandsubgenomicRNAsspondingORFsinbovinecoronavirus(BCV)and(Holmes&Lai,1996).humancoronavirus(HCV)0C43revealeds
6、equenceHEVbelongstotheantigenicgroupIIofcoronaviruses,similaritiesofgreaterthan88.5%atthenucleotidetogetherwithbovinecoronavirus(BCV),humancoronavirusand85.3%attheaminoacidlevelforthe12.8kDa(HCV)strainOC43,turkeycoronavirus(TCV)andmouseORFproduct.Fort
7、he9"6kDaORFproductsimi-hepatitisvirus(MHV).Membersofthisgrouppossessfivelaritiesweregreaterthan96-9%and95-2%,structuralproteins,nucleocapsid(N),membrane(M),smallrespectively.Anadditional12nucleotidedeletionmembrane(sM),spike(S)andhaemagglutinin-estera
8、se(HE),upstreamofthe12-8kDaORFstartcodonwaswhichareencodedbymRNAs7,6,5"1,3and2"1,respectively.foundinHEV67NcomparedtoNT9andVW572.Additionally,ORFspotentiallyencodingnon-structural(ns)TheseresultsrevealagenomicorganizationofHEVproteinshavebeeni
