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1、178Mucosalimmunity23June1997-PosterpresentationsResulte:Therehavebeenhtthpronouncedblast-celltransformationregard-duction,thecapacityofmucosalTlymphocytestoexpressmRNAforIL10,IFNyingtoautologoussalivainallpatientsandthehighestlevelincancerpersons.andLMIF(leucocytemigrationinhibitionfactor)isex
2、aminedinthisstudy.RNAThemitogeneticeffectofpatientssalivatoheterologouscellsinhealthypersonswasextractedfromwholeintestinalbiopsiesandfromsinglecellsuspensionsofhasalsobeenreliablemorethaninacontrol.epithelialandlaminaproptfafractionsfollowingtreatmentwithEDTA/DlTandConclusion:Atpresentmitogen
3、eticfactorsinsalivaresultingintheproliier-collagenase.RTPCRwasperformedusingcytokinespecificprimersontheativeresponseoflymphocytesarentquitecharactetized.Perhaps,thehighestextractedRNA.Inaddition,CD3+Tcellswereisolatedfromthelaminapmprtalevelsofsuchactivitymightcorrespondtothemostseriousdisord
4、ersoforalandfromtheepitheliallayers(intraepitheliallymphocytes-IELs)usingmagneticlocalimmunity.beadscoatedwithantiCD3.RTPCRfortheabovecytoldneswasperformedonoliaodTcoatedmaoneticbeadsfollowtnaextractionofmRNAfromIELsandfromkminapropriaTcells.P.5.08.08TGEV-specificIgAatdifferentmucosaefollowing
5、Reeutts~TheresultsdemonstratetheexpressionofmRNAforLMIFinwholeInfectionofpigswithtransmissiblegastroenterltlsintestinalbiopsies,inthefractionatedepithelialandlaminapmptialayersandvirusortheantlgenlcaliyrelatedporcineinisolatedIELandlaminapmprtaTcellpopulations.TheexpressionofmRNArespiratorycor
6、onavirusforIL10wasobservedinwholebiopsiesin2/5subjectsandwasalsodetectedinboththeepithelialandlaminapmptiafractions.mRNAexpressionforIFNyE.Cox’,MB.Pensaert*,B.M.Goddeeds.Lab.Vet./mmuno/.,Departmentwasfoundin4/5wholebiopsiesandincellsuspensionsofboththeepithelialofVirvl~Pam&o/~,Immunol~~Fat.Vet
7、Med.,RUG,Mereibeke,andlaminapmprtafractions.Belgium,*Lab,Vet.Vim/.,DepartmentofViroiog~Parasitolog)!Immundogy;Conclusions:ThisstudydemonstratestheuseofmagneticbeadstoextractFat.Vet.Med.,RUG,Merelbeke,Belgiumsoecificcelloooulationsfromsm
