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1、ArchivesofVirologyArchivesofVirology68,45--52(1981)©bySpringer-Verlag1981AnImmunoeleetronMicroscopicandImmunofluoreseentStudyontheAntigenicRelationshipBetweentheCoronavirus-LikeAgent,CV777,andSeveralCoronavirusesByM.B.PENSAERT1,P.DEBOUCK1,andD.J.t~EYNOLDS21LaboratoryofVirology,FacultyofVeterinar
2、yMedicine,StateUniversityofGent,Gent,BelgiumAgriculturalIgesearchCouncil,InstituteforResearchonAnimalDiseases,ComptonNr.Newbury,Berkshire,GreatBritainWith2FiguresAcceptedNovembert8,t980SummaryApossibleantigenicrelationshipbetweentheporcineenteropathogeniccorona-virus-likeagent,(CVLA)and6knowncor
3、onaviruseswasexaminedbyimmuno-electronmicroscopy(IEM)andbyimmunofluorescenee(IF).CVLAdidnotshowcrossreactivitywithinfectiousbronchitisvirus,transmissiblegastroenteritisvirus(TGEV),canineeoronavirus(CCV)hemagglutinatingencephalomyelitisvirus(HEV),neonatalcalfdiarrheacoronavirus(NCDCV)orfelineinfe
4、ctiousperitonitisvirus(FIPV).AntigenicrelationshipwasdetectedbyIEMbetweenTGEVandCCV,NCDCVandI-IEVandbyIFbetweenTGEVandCCV,TGEVandFIPV,HEVandNCDCV.IntroductionInthethirdreportoftheInternationalCommitteeonTaxonomyofViruses,thefamilyCoronaviridaeiscitedtocontain5definitemembers,5probablemembersand2
5、possiblemembers(8).The12speciesofthismonogeneriefamilyaregroupedmainlyonphysieo-chemiealcharacteristics.Theyarepleomorphieenvelopedparticles,averaging10nmdiameter,containingRNAandessentiallipid.Theyallbearuniquedefiniteprojections.Theeoronavirusesmultiplyincytoplasmandmaturebybuddingthroughendop
6、lasmicretieulum(18).Someeoronavirusescauserespiratoryproblemsinbirdsandman,othersareassociatedwithenteritisindifferentspeciesandsomeeoronavirusesaffectmultipleorgans(13).Antigenicrelationshipsexistonlybetweensomemembersofthisfamily(i8).In1977,acoronavirus-likeagent(CVLA)wasisolatedfromanepizooti
7、cofdiarrheaonBelgianswinebreedingfarms(14).Basedonitsmorphologiccharacter-istics,theCVLAwassuggestedtobeatentativememberofthefamilyCorona-0304-8608/8t/0068/0045/$0i.6046M.B.PENSAERT,P.DEBOUCK,andD.J.REYNOLDS:viridae(4).TheCV
