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1、T细胞疫苗诱导异种胰岛移植免疫耐受实验探究［摘要］目的体外制备供体特异性T细胞疫苗(Tcellvaccine),探讨T细胞疫苗免疫(Tcellvaccination)诱导异种胰岛细胞移植免疫耐受的作用。方法腹腔注射链淼佐菌素诱导建立Balb/c小鼠I型糖尿病模型，用酶消化法和密度梯度离心法无菌分离纯化Wistar大鼠胰岛细胞。制备Balb/c小鼠针对Wistar大鼠的T细胞疫苗：实验随机分为三组,G1：糖尿病小鼠对照组;G2：胰岛细胞移植组，糖尿病小鼠+胰岛细胞移植+1640培养液(对照组)；G3：胰岛细胞移植加T细胞疫苗免疫组，糖尿病小
2、鼠+胰岛细胞+T细胞疫苗(实验组)，于dl,d7,dl4,d21实验组Balb/c小鼠接种TCV(lX107/mL),对照组用1640替代。接种前和每次接种后第5d分别进行混合淋巴细胞反应。胰岛细胞移植：将Wistar大鼠的胰岛按1200〜1500IEQ/kg经肝门静脉导入小鼠体内，移植后观测血糖和体重变化。结果异种胰岛细胞移植可以诱导I型糖尿病小鼠血糖降至正常水平且维持一段时间，对照组小鼠移植后(5.12±1.36)d即发生排斥，实验组移植物存活时间达(20.25±3.45)do结论TCV能够有效延长异种胰岛移植物的存活时间，是一种潜在
3、诱导异种胰岛移植免疫耐受的方法。［关键词］T细胞疫苗;胰岛细胞;异种移植;免疫耐受［中图分类号］R349.1［文献标识码］A［文章编号］1673-9701(2010)04-19-03TheEffectsofTCellVaccinationonXenogeneicIsletCellTransplantationHUChunleilZHAOZhenlin21.ShanxiMedicalUniversity,Taiyuan030001,China；2.TheSecondHospitalofShanxiMedicalUniversity,Taiy
4、uan030001,China［Abstract］ObjectiveToexplorethetheeffectsofTcellvaccinationonxenogeneicisletcelltransplantation.MethodsBalb/cmice(H2~d)wereusedasrecipientsandWistarratswereusedasdonors・TypeIdiabetesmicemodelweremadebyinjectingstreptozotocinintravenously.TypeIdiabetesmicewe
5、rerandomLydividedintothreegroups,GroupA：DiabeticmicewithnotransplantationandnoTCV,GroupB：Isletcelltransplantation+RPM11640culturemedium,GroupC(experimentalgroup):Isletcelltransplantation+TCV.TcellvaccinewasmadebyusingattenuatedspleenlymphocytesfromBalb/cmicewhichwerechall
6、engedwithWistarratspleenlymphocytesinvitroandwerestimu1atedwithConA.Balb/cmiceasrecipientswereimmunizedwithTCVonthetimepointsofdl,d7,dl4,d21and；RPM11640culturemediumwasusedincontrolgroup.One-waymixedlymphocytereaction(MLR)wasperformedwitheffectorofimmunizedBalb/cmiceperip
7、hericlymphocytesandstiinulatorofWistarratperiphericlymphocytesontheseconddayofTcellvaccinatiori.IsletcellswereisolatedfromWistarratpancreasusingtheprotocolofenzymedigestionanddensitygradientcentrifugation.IsletcellsweretransplantedintoimmunizedBalb/cmiceatthedensityof1200
8、〜1500IEQ/kgbythewayofportalveininjecticrn.Recipient?sbloodglucosewasmonitoredeverydayandbodyweig
