BG11液体培养配方.doc

《BG11液体培养配方.doc》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、BG11液体培养基就是：Stock1定容100mL柠檬酸0.3g柠檬酸铁胺0.3gEDTANa20.05gStock2定容1000mLNaNO330gK2HPO40.78gMgSO4·7H2O1.5gStock3定容100mLCaCl2·2H2O1.9gStock4定容100mLNa2CO32gStock5定容1000mLH3BO32.86gMnCl2·4H2O1.81gZnSO4·7H2O0.222gNa2MnO4·2H2O0.391gCuSO4·5H2O0.079gCo(NO3)2·6H2O0.049gStoc

2、k1取用2mLStock2取用20mLStock3取用2mLStock4取用1mLStock5取用1mL总定容1000mLD1(Diatommedium) Component            Amount        StockSolution    (1)NaNO3                  1mL/L            12g/100mldH2O         (2)K2HPO4                 1mL/L            4g/100mLdH2O        (3

3、)MgSO4·7H2O         1mL/L            7g/100mLdH2O        (4)CaCl2·2H2O          1mL/L            2g/100mLdH2O      (5)KH2PO4                1mL/L            8g/100mLdH2O       (6)Na2SiO3.9H2O        1mL/L           10g/100mldH2O(7)MnSO4                 0.1mL/L

4、            0.2g/100mldH2O(8)柠檬酸铁                1mL/L             0.5g/100mldH2O(9)A5（Tracementalsolution） 1ml/L                 H3BO3                     2.86g/LdH2O         MnCl2·4H2O              1.86g/LdH2O         ZnSO4·7H2O             0.22g/LdH2O      

5、   Na2MoO4.2H2O            0.39g/LdH2O         CuSO4.5H2O              0.08g/LdH2O         Co(NO3)2.6H2O           0.05g/LdH2O(10)土壤提取液              20mL/L 土壤提取液配制方法:取花园土未施过肥200g置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热3小时，冷却，沉淀24小时，此过程连续进行3次，然后过滤，取上清液，于高压灭菌锅中灭菌

6、后于4℃冰箱中保存备用。 f/2 mediumComponent                          Amount          (1) NaNO3                      75mg/L(2) NaH2PO4·2H2O         0.6mg/L(3) VitaminB12                0.5μg/L(4)  Biotin                       0.5μg/L(5) ThiamineHCl              100μg/L(6)

7、 Na2SiO3·9H2O          10mg/L(7)  f/2metals                 1mL/L       Na2EDTA·2H2O     4.4g       FeCl3·6H2O        3.16g       CoSO4·7H2O       0.012g       ZnSO4·7H2O       0.021g       MnCl2·4H2O         0.18g       CuSO4·5H2O       0.007g      Na2MoO4·2H

8、2O      0.007g       Distilledwater        1000mL(8)   Seawater                        1000mLBG-11是培养蓝藻使用最广泛的培养基，微量元素溶液A5是BG-11培养基微量元素的重要组成部分。    试剂名称g/L 备注NaNO31.5g  K2HPO40.04g