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1、BioresourceTechnology100(2009)5922–5927ContentslistsavailableatScienceDirectBioresourceTechnologyjournalhomepage:www.elsevier.com/locate/biortechMediumoptimizationfortheproductionofanovelbioflocculantfromHalomonassp.V3a0usingresponsesurfacemethodologya,1a,1aabba,*JinHe,QuanweiZh
2、en,NingQiu,ZiduoLiu,BaojiangWang,ZongzeShao,ZiniuYuaStateKeyLaboratoryofAgriculturalMicrobiologyandNationalEngineeringResearchCenterofMicrobialPesticides,HuazhongAgriculturalUniversity,Wuhan430070,PRChinabThirdInstituteofOceanography,StateOceanicAdministration,Xiamen,361000,PRC
3、hinaarticleinfoabstractArticlehistory:ThenovelexopolysaccharidebioflocculantHBF-3isproducedbyHalomonassp.V3a0,whichisamutantReceived19March2009strainofthedeep-seabacteriumHalomonassp.V3a.Responsesurfacemethodology(RSM)wasemployedReceivedinrevisedform4June2009tooptimizetheproduct
4、ionmediumforincreasingHBF-3production.UsingaPlackett–Burmanexperi-Accepted5June2009mentaldesigntoaidinthefirststepofoptimization,edibleglucose,MgSO47H2O,andNH4ClwerefoundAvailableonline24July2009tobesignificantfactorsaffectingHBF-3production.Todeterminetheoptimalconcentrationofe
5、achsig-nificantvariable,acentralcompositedesignwasemployed.BasedonresponsesurfaceandcanonicalKeywords:analysis,theoptimumconcentrationsofthecriticalcomponentswereobtainedasfollows:edibleglu-Halomonassp.V3a0BioflocculantHBF-3cose,16.14g/l;MgSO47H2O,2.73g/l;andNH4Cl,1.97g/l.HBF-3p
6、roductionobtainedbyusingtheopti-mizedmediumwas4.52g/l,whichwasincloseagreementwiththepredictedvalueof4.55g/l.ByscalingMediumoptimizationPlackett–Burmandesignupfermentationfromflasktofermenter,HBF-3productionwasfurtherincreasedto5.58g/l.ResponsesurfacemethodologyÓ2009ElsevierLtd.
7、Allrightsreserved.1.Introduction2002;Gangadharanetal.,2008),metabolitesecretion(WangandWan,2008;Bajajetal.,2009).Comparedwithconventionalmeth-Microbial-producedbioflocculantshavereceivedincreasedsci-ods,whicharetime-consumingbecausetheyrequirealargenum-entificandtechnicalattentio
8、nbecausetheyarebiodegradableandberofexperimentstodeter
