Production and characterization of an intracellular bioflocculant by.pdf

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1、BioresourceTechnology101(2010)1044–1048ContentslistsavailableatScienceDirectBioresourceTechnologyjournalhomepage:www.elsevier.com/locate/biortechProductionandcharacterizationofanintracellularbioflocculantbyChryseobacteriumdaeguenseW6culturedinlownutritionmediumWeiJieLiu,KaiWang,BaoZhenLi,H

2、ongLiYuan,JinShuiYang*StateKeyLabforAgrobiotechnology,CenterofBiomassEngineering,KeyLaboratoryofAgro-MicrobialResourceandApplication,MinistryofAgriculture,CollegeofBiologicalSciences,ChinaAgriculturalUniversity,Beijing100193,ChinaarticleinfoabstractArticlehistory:Anovelintracellularbiofloc

3、culant(namedMBF-W6)producedbyChryseobacteriumdaeguenseW6cul-Received25June2009turedinlownutritionmediumwasinvestigatedinthisstudy.Theeffectsofcarbonsource,nitrogenReceivedinrevisedform28August2009source,C/Nratio,initialpH,inoculumsize,culturetemperatureandshakingspeedonMBF-W6produc-Accept

4、ed28August2009tionwerestudied.ChemicalanalysisshowedthatthepurifiedMBF-W6wasmainlycomposedof32.4%Availableonline18September2009protein,13.1%polysaccharideand6.8%nucleicacid.Fourier-transforminfrared(FTIR)spectroscopyindi-catedthepresenceofcarboxyl,hydroxyl,andmethoxylgroups.Theelementalana

5、lysisofpurifiedMBF-Keywords:W6revealedthatthemassproportionofC,H,O,NandSwas40.92:6.53:44.01:8.53:1.01(w/w)corre-Chryseobacteriumdaeguensespondingly.MBF-W6hadgoodflocculatingrateinKaolinsuspensionwithoutanycationaddition.TheFlocculatingrateFlocculanthighestflocculatingrateof96.9%wasachievedun

6、dertheoptimalconditions(bioflocculantdosage1Intracellularbioflocculant1.2mgl,pH5.6andtemperature15°C).ExtracellularpolymericsubstancesÓ2009ElsevierLtd.Allrightsreserved.1.IntroductionManyparticularmicroorganismsandtheirbioflocculantshavebeenreported.Zhengetal.(2008)discoveredabioflocculantpr

7、o-Atpresent,flocculatingagentsareprevalentinvariousindus-ducedbyBacillussp.F19culturedinmediumcontainingsucrose11trialprocessessuchaswastewatertreatment,drinkingwaterpuri-20glandyeastextract2.5gl.Thisbioflocculantwasmainlyficationanddownstreamprocessesinfoodandfermen