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1、Availableonlineatwww.sciencedirect.comBioresourceTechnology99(2008)7686–7691ProductionandcharacteristicsofabioflocculantproducedbyBacillussp.F19YanZheng,Zhi-LongYe,Xu-LiangFang,Ya-HongLi,Wei-MinCai*SchoolofEnvironmentalScienceandEngineering,ShanghaiJiaoTongUniver
2、sity,Shanghai200240,ChinaReceived29October2007;receivedinrevisedform29January2008;accepted30January2008Availableonline20March2008AbstractAbioflocculant-producingbacteriumisolatedfromsoilwasidentifiedasBacillussp.andthebioflocculantproducedwasnamedMBFF19.Effectsofphy
3、sico-chemicalconditionsincludingpH,carbonsourcesandnitrogensourcesonMBFF19productionwerestud-ied.ChemicalanalysesofthepurifiedbioflocculantMBFF19indicatedthatitwasasugar-proteinderivative,composedofneutralsugar(3.6%,w/w),uronicacid(37.0%,w/w),aminosugars(0.5%,w/w)
4、andprotein(16.4%,w/w).Thetwoneutralsugarcomponentsweremannoseandglucoseandthemolarratiowas1.2:1.InfraredspectrophotometryanalysisrevealedthatMBFF19containedcarboxyl,hydroxylandmethoxylgroupsinitsstructural.FlocculatingpropertiesofbioflocculantMBFF19wasexaminedusi
5、ngkaolin,activated3+carbonandflycoalsuspension.CationsupplementhadnopositiveeffectsontheflocculatingactivitywhereasthepresenceofFeinhib-itedflocculation.InfluencesofpHandbioflocculantdosageontheflocculationwerealsoexamined.Ó2008ElsevierLtd.Allrightsreserved.Keywords:Ba
6、cillussp.;Bioflocculant;Flocculation;Polysaccharide1.Introductionetal.,2001)becauseoftheirbiodegradabilityandsafetyforecosystems(Heetal.,2004).Atpresent,flocculantsareprevalentinthevarietyofBioflocculants,secretedbyalgae,bacteria,fungiandindustrialprocessessuchaswa
7、stewatertreatment,drinkingyeast,arekindsofextracellularbiopolymerincludingwaterpurificationanddownstreamprocessesinfermenta-proteins,glycoproteins,polysaccharides,lipidsandglyco-tionprocesses(Shihetal.,2001).Althoughchemicallysyn-lipids(SalehizadehandShojaosadati
8、,2003).Manyparticu-theticflocculantsareplayingdominantrolesassociatedlarmicroorganismsandtheirbioflocculantshadbeenwiththeireffectivenessandlowcost,theyarehardannouncedl
