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时间:2020-02-29
《烟管菌T1原生质体制备及菌丝再生条件优化 优先出版.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、DOI:10.13989/j.cnki.0517-6611.2017.11.001网络出版时间:2017-05-0408:33:21ST网络出版地址:UVWX,Journalhttp://kns.cnki.net/kcms/detail/34.1076.S.20170504.0833.002.htmlofAnhuiAgri.Sci.2017,45(11):1-2,38!"#T1$%&'()*#+,%-./0!"#,$%&,'(),*+,(!"#$%&'()&&*,!"+,230036)12[-.]/0123T1(Bjerkanderaadusta)456789:3;<5.=>?@。
2、[AB]/03C、DEFGHIJKLMN、pH、OMN:OPQRS4567TU.VW,X/0<5YZ[JKS3;<5.VW。[]]^PDA_7YZ[`,a28℃bcYZ3d.3;=def3456789;g0.5mol/LKClh8.ij15mg/mL.klOe30℃mOP3h,nT4op=!,q6118×10r/mL;RM1YZ[<5U=!,q1.22%。[s]f/0tuv456789:3;<5?@,wxyz/0123{
3、}~8s。345123T1;456789;3;<5;tu6789:S182;<=>?A;@A:0517-6611(2017)11-0001-02Protopl
4、astPreparationandHyphaeRegenerationConditionOptimizationofBjerkanderaadustaT1GAOXueyan,CHUQiaoling,ZHOULingfeng,HANGuomin(SchoolofLifeSciences,AnhuiAgricultureUniversity,Hefei,Anhui230036)Abstract[Objective]TostudyprotoplastspreparationandhyphaeregenerationconditionsofBjerkanderaadusta.[Me
5、thod]Theeffectofdifferentcultureconditions(hyphaeage,stabilizerandstabilizerconcentration,pH,enzymaticconcenfration,enzymatichydrolysistime)onprotoplastspreparationandhyphaeregenerationconditionsofBjerkanderaadustawasstudied.[Result]ThefungalmyceliaculturedintheliquidPDAmediumat28℃for3dayswas
6、themostsuitableforprotoplastpreparation.15mg/mLsolublewallenzymein0.5mol/LKClhydrolyzingfor4hours6couldgenerateasmanyas118×10protoplasts/mL.TheRM1mediumwasthepreferredregenerationmediumonwhichtheregenerationratewasashighas1.22%.[Conclusion]Thestudyoptimizetheconditionsofprotoplastspreparationa
7、ndregeneration,andprovidetheorybasisforfurtherstudyofmolecularmechanismofBjerkanderaadusta.KeywordsBjerkanderaadustaT1;Protoplastpreparation;Hyphaeregeneration;Optimization-./,012345/567,89、:9、;<、=>¿Öf®'ôõßö÷ø,ù¢÷2Õ'bl¿Öfx2?@9、AB2CD2E、FB2GH@I9JKLM2®'ôõ。[1-3]NHOP0QRSTUVWX2YZ01。01[1NOPQR]%^2_`ab
8、———Z`c,de0'fg''ah`,1.1NO[8]ijklVm、jn、opqJ,WXrskt'u。@vw1.1.1x©ÆOúûï。ÁÂxT1§üýþ¶·¸,xxyz{
9、}~YZ-.4U22~Zÿè!ü"#$%'a×Ø。[3-4][5-6]。JiaJ4Uwxx———1302BG1.1.2ðñò。PDAlðñò:&'(200g/L,)*+(Trichaptumabietinum)xlulZz{
10、20g/L;PDA,lðñò:&'(200g/L,)*+20g
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