HE切片褪色后行EBER原位杂交检测方法的探讨.pdf

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1、临床和实验医学杂志2017年1月第16卷第1期·101·ACSInfectDis，2016，2(2):132－139．［16］MehtaY，JaggiN，ＲosenthalVD，etal．Effectivenessofamultidimen-［15］LeblebiciogluH，ErsozG，ＲosenthalVD，etal．Impactofamultidi-sionalapproachforpreventionofventilator－associatedpneumoniain21mensionalinfectioncontrolapproachoncatheter－ass

2、ociatedurinaryadultintensive－careunitsfrom10citiesinIndia:findingsoftheInter-tractinfectionratesinadultintensivecareunitsin10citiesofTurkey:nationalNosocomialInfectionControlConsortium(INICC)［J］．Epide-InternationalNosocomialInfectionControlConsortiumfindings(INICC)miolInfect，2013，141(12):24

3、83－2491．［J］．AmJInfecControl，2013，41(10):885－891．(收稿日期:2016－09－23)DOI:10．3969/j．issn．1671－4695．2017．01．034文章编号:1671－4695(2017)01－0101－03HE切片褪色后行EBEＲ原位杂交检测方法的探讨滕孝静梅雪杨秀静王照情孟凌轩王凤(首都医科大学附属北京友谊医院病理科北京100050)【摘要】目的探讨将HE切片褪色后行EB病毒编码的小ＲNA(EBEＲ)原位杂交检测的方法。方法收集40例EBEＲ原位杂交检测阳性病例的HE切片作为实验组，相同蜡块的连续切片作为对照组

4、，分别进行EBEＲ原位杂交检测。结果实验组中，38张切片的组织结构全部完整，2张骨髓穿刺切片中的骨小梁脱片，但剩余组织组织结构保持完整。所有40张切片均杂交阳性，阳性信号位于细胞核，呈棕黄色，定位准确、清晰可读，杂交背景干净。阳性细胞数目及强度与对照组无差别。结论将HE切片褪色后行EBEＲ原位杂交检测，方法简单、可靠，在病理诊断中有一定的应用价值。【关键词】HE染色褪色EBEＲ原位杂交StudyonthetechniqueofEBEＲinsituhybridizationonHEstainingsectionsafterdecoloration．TENGXiao－jing，M

5、EIXue，YANGXiu－jing，etal．DepartmentofPathology，BeijingFriendshipHospital，CapitalMedicalUniversity，Beijing100050，China．【Abstract】ObjectiveToexplorethetechniqueofEBEＲinsituhybridizationonHEstainingsectionsafterdecoloration．MethodsFor-tycasesofEBV－positiveexpressionweretestedinthisstudy．OneHEse

6、ctionandonesequentialsectioncutfromthesametissueblockofeachcasehadbeenallottedintotwogroups:trialgroup(HEsections)andcontrolgroup(sequentialsections)．Insituhybridizationhadbeenper-formedinbothgroupsrespectively．ＲesultsIntrialgroup，38outof40sectionsshowedintacttissuestructurewhileontheleft2s

7、ectionsofbonemarrowbiopsy，bonetrabeculadetachedfromtheslide．Butallthese40sectionswerepositiveforEBEＲinsituhybridization．Thepositivesignalwaslocatedatthenucleusasbrownishyellow．Thesignallocationwasaccurateandcoloringresultswerediscernible．Nobackgroundinte