Brivanib对HepG2细胞的抑制作用及机制 优先出版.pdf

《Brivanib对HepG2细胞的抑制作用及机制 优先出版.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、现代肿瘤医学2017年02月第25卷第03期MODEＲNONCOLOGY，Feb.2017，VOL.25，NO.03·355·Brivanib对HepG2细胞的抑制作用及机制12韩兴华，王章桂InhibitoryeffectandmechanismofBrivanibonHepG2cells12HanXinghua，WangZhanggui12DepartmentofOncology，TheAffiliatedProvincialHospitalofAnhuiMedicalUniversity，AnhuiHefei230001，China;Departmento

2、fＲa-diationOncology，TheSecondPeople＇sHospitalofAnhuiProvince，AnhuiHefei230001，China．【Abstract】Objective:ToexploretheinhibitoryeffectandmechanismofBrivanibonhumanhepatocellularcarci-nomaHepG2cells．Methods:Theanti－proliferativeeffectofBrivanibonHepG2cellswereassessedbyMTS．HepG2cellapop

3、tosisratewasdeterminedbyflowcytometry(FCM)analysisaftervariousconcentrationBrivanibtreatment．TheexpressionsofBcl－2andBaxweredetectedbyWesternblot．TheexpressionofLC3wereexaminedbyimmuno-fluorescenceandWesternblot．Ｒesults:Theanti－proliferativerateswereincreasedindose－dependantandtime－d

4、ependantmanners．AfterincubatedwithdifferentdosesofBrivanib，theinhibitoryratesofHepG2cellswere(13．06±4．06)%，(20．89±1．83)%and(44．29±2．56)%，respectively．Theapoptosisrateswere(13．06±4．06)%，(20．89±1．83)%and(44．29±2．56)%，respectively．WhichwerecorrelatedwiththedecreasingofBaxandin-creasingo

5、fBcl－2．Furthermore，theexpressionofLC3wasincreasedby2．5μmol/LBrivanibtreatment，andp62de-creased．Conclusion:BrivanibinhibitstheproliferationofHepG2cells，inducesapoptosisandactivatesautophagy．【Keywords】hepatocellularcarcinomacells，Brivanib，apoptosis，autophagyModernOncology2017，25(03):03

6、55－0357【摘要】目的:探讨Brivanib对人HepG2肝癌细胞的增殖抑制作用及其机制。方法:MTS法检测Brivanib对HepG2细胞的增殖抑制作用，流式细胞仪检测不同浓度Brivanib处理后HepG2细胞的凋亡率，Westernblot检测凋亡相关蛋白Bcl－2、Bax的表达情况，免疫荧光观察Brivanib处理后HepG2细胞内源性LC3表达情况，Westernblot检测自噬关键蛋白LC－I向LC－II的转换及p62的表达。结果:与空白对照组相比，Brivanib对HepG2肝癌细胞的增殖抑制率随剂量增加和时间延长而增加，呈剂量和时间依赖性。不

7、同浓度Brivanib作用72h后HepG2细胞的凋亡率分别为(13．06±4．06)%、(20．89±1．83)%、(44．29±2．56)%，其诱导凋亡与下调Bax、上调Bcl－2表达相关。2．5μmol/LBrivanib处理HepG2细胞48h后内源性LC3表达增加。Westernblot分析表明，LC3－I向LC3－II转换增加，p62表达降低。结论:Brivanib抑制HepG2人肝癌细胞增殖，诱导凋亡和自噬活化。【关键词】肝细胞癌;Brivanib;凋亡;自噬【中图分类号】Ｒ735．7【文献标识码】ADOI:10．3969/j．issn．1672－

8、4992．2017．03