19 病毒对先天性心脏病先天感染的探证及电镜观察.doc

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4、OBH?。-eopo一一一一••〜kf><••y/"••••/X••••••✓IAA££^O2i£>I,°UJE^pAEd°；・^aAUB192;SI^giOIEiiDODAda2;pAdBE^ipIEiiDdADE^EudA£^2jS[^»udd£EAUduoIOUEPOvI,ou°E♦♦✓AU・iijjKeywords:congenitalheartdisease;parvovirusBl9;mi-croscopyE-'electroniijjAbstract:AIMTotestiftheparv

5、ovirusB19infectionincar-diactissueincongenitalheartdiseaset^CHDEOiscongenital—andtoobservetheultrastructuralfeaturesofcardiactissueofCHD.METHODSByusingELISA£-'B19-VP2-1gM;jwasdetectedinperipheralbloodL^andusingPCRandTSHL^Bl9-DNAwasdetectedincardiactissue

6、from42pa.tientswithcongenitalheartdiseaseand22healthcontrols：ultrastruc-turewasobservedbyelectronmicroscopyforB19-DNAposi-tivecardiactissueofCHD.RESULTSUB19~TgMpositiverateswere4.7%and4.l%inperipheralbloodofCHDandcontrols£_irespectively・Therewasnosignifi

7、cantdifferencet••P>0.05£©・<^UB19一DNApositiverateswerel9・l%andO%incaTdiactissueofCHDandcontrolrespectively.Nosignificantclifferencewasseen£，*卩&It;0.OlEQ—andCCdBig-DNAwaslocatedincardiaccel1nuclearbyTSHt-'butultrastructureofBl9~DNApositivecardiactissuef

8、oundneitherviralparticlesinnucleusandcytoplasmnorlesionofcel1organbytransmissionelec-tronmicroscope・CONCLUSIONInfectionofparvovirusB19isconfirmedtobecongenitaiandBl9-DNAmightbeintegart~edwithnucleicacidwithinhostcel1.iiiiO