19病毒对先天性心脏病先天感染的探证及电镜观察

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5、ijj;Keywords:congenitalheartdise8se;parvovirusB19;mi-croscopy£-«electronj;jjAbstract:AIMTotestiftheparvovirusB19infectionincar-diactissueincongenitaiheartdiseaseL^CHDE©!scongenitalEiandtoobservetheultrastructuralfeaturesofcardiactissueofCHD.METH0DSByusingELISA£_iB19-VP2-I

6、gMjjwasdetectedinperipheralbloodndusingPCRandISH£「Bl9-DNAwasdetectedincardiactissuefrom42patientswithcongenitalheaTtdiseaseemd22healthcontrols;ultrastruc-turewasobservedbyelectronmicroscopyforB19-DNAposi-tivecardiactissueofCHD.RESULTSCuB19_TgMpositiverateswere4.7%and4.l%i

7、nperipheralbloodofCIIDandcontrolsfrrespectively.Therewasnosignificantdifference£_P>0.05£©.uB19~DNApositiverateswerel9・l%andO%incaTdiactissueofCHDandcontrolrespectively.Nosignificantdifferencewasseen£,,P;0.OlEQ—andcCfBig-DMAwaslocatedincardiaccel1nuclearbylSHfrbutiiltra

8、stmctureofB19~DNApositivecardiactissuefoundneitherviralparticlesirrnucleusandcytoplasmnorlesionofcellorganbytransniissionelec-tronmicroscope・CONCLUSIONInfectionofparvovirusB19isconfirmedtobecongenitalandBig—DNAmightbeintegart—edwithnucleicacidwithinhostcel1.iiiiOOyNOijijj

9、2jSI^BigE^ParvovirusBlOLOEQ^ulOOaAeA・^IOpAOeEEAa^22;AUQDIa10pA2jO-Ia£^IbONO^EpL^EuiEdOdy^S,DE^Dd