荧光素酶报告基因检测系统原理.ppt

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1、TheCentralMelanocortinSystemCanDirectlyRegulateSerumInsulinLevelsReporter：ZiqiZhangAuthor:RogerD.Coneetal.From:Endocrinology(2000)2015/6/22点击添加文本点击添加文本点击添加文本点击添加文本BackgroundMethodprocessConclusionContents点击添加文本点击添加文本点击添加文本点击添加文本BackgroundObesityHyperinsulinemiaInsulin

2、resistanceType2diabetesThemechanisticrelationshipbetweenobesity,hyperinsulinemia,insulinresistance,anddiabetesisnotentirelyclear.点击添加文本点击添加文本点击添加文本点击添加文本AvyMiceectopicallyexpressingagoutihyperphagia,hyperinsulinemic,hypoactivity,andobesityMiceoverexpressingthehypothal

3、amicAGRPhyperphagia,severeobesity,hyperinsulinemia,late-onsethyperglycemia,MicelackingPOMC–derivedpeptidehyperphagiaandobesityandinsulinresistanceMicelackingMC4Robesity,insulinresistance,anddyslipidemia.BackgroundMousemodelsHyperinsulinemiaisoneofcommonfeaturesamongal

4、ltheanimalmodelsofobesity,andalsooneoftheearlistmetabolicalterationsobservedinmodels.Thedevelopmentissimilartotheprocessoftype2diabetes点击添加文本点击添加文本点击添加文本点击添加文本TheCentralMelanocortinsystemEnergyintakeEnergyexpenditureGlucosehomeostasisBackgroundMC4RMC3RHypothesis:thece

5、ntralmelanocortinsystemmaybeinvolvedintheregulationofglucosehomeostasis点击添加文本点击添加文本点击添加文本点击添加文本GlucosehomeostasisBackground外周组织对胰岛素的敏感性点击添加文本点击添加文本点击添加文本点击添加文本中央黑皮质素信号传导缺陷（未知）肥胖组织增生干扰胰岛素作用（有报道）Background其他未知瘦素缺陷（有报道）胰岛素敏感性下降血糖升高胰岛补偿性分泌胰岛素增加血糖失衡血液胰岛素增加高胰岛素血症胰岛素抗性血糖下降暂时

6、性血糖平衡糖尿病点击添加文本点击添加文本点击添加文本点击添加文本Objective1.Centralmelanocortinreceptorsignalingmayplayaroleinthecontrolofglucosehomeostasis.2.Ahypothesis:themelanocortinagonistlowersseruminsulinbystimulatingthesympatheticdrivetothepancreasknowntoinhibitinsulinrelease.3.Whetherthehype

7、rinsulinemiainthemelanocortinobesitysyndromesisprimarytoMC4Rblockade,adevelopmentconsequenceofMC4Rblockade,orsecondarytotehyperphagiaandobesity.点击添加文本点击添加文本点击添加文本点击添加文本MethodSurgicalprocedureRIAassayforserumorplasmainsulinBloodglucoseandglucosetolerancetestNonesterifi

8、edfattyacidassayInsulintolerancetest点击添加文本点击添加文本点击添加文本点击添加文本Process1Ob/obmouseIcvinjectionofMTⅡ(0.3、1、3nmol)、ACSF、LeptinAfte