发育生物学_embryonic stem cell_F1.ppt

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1、StemCellByWei-JunCaiDept.ofHistology&EmbryologySchoolofXiangyaMedicine,CSU1978-test-tubebabyEmbryonicStemCellHistolyHowdoesthisoccurandwhatarethecellularandmolecularmechanisms?Howdoesasinglecelldevelopintoabody?LouiseBrownSirMartinEvanshasrecentlybeenhonoredwiththeNobelPrizeforPhysi

2、ologyandMedicine(2007)forhiscontributiontowardsdevelopmentofanimalmodelsofdiseasethroughESCmediatedgenetargeting.SirMartinEvansreceivingtheNobelprize1981-EvansandKaufumanfirstextractstemcellsfrommouseembryosEmbryonicStemCellHistolyNature.1981Jul9;292(5819):154-6.Developindefinitelyi

3、nculture,normalkaryotype1995-fromanon-humanprimaterhesusmonkeyembryos1998-firstextractstemcellsfromhumanembryosEmbryonicStemCellHistolyJamesThomsonIn1998,JamesThomson(UniversityofWisconsin-Madison)isolatedcellsfromtheinnercellmassoftheearlyembryo,anddevelopedthefirsthumanembryonicst

4、emcelllines.In1998,JohnGearhart(JohnsHopkinsUniversity)derivedhumanembryonicgermcellsfromcellsinfetalgonadaltissue(primordialgermcells).Pluripotentstemcell“lines”weredevelopedfrombothsourcesWhatAreEmbryonicStemCell?Embryonicstemcells(EScells)arepluripotentstemcellsderivedfromtheinne

5、rcellmassoftheblastocyst,anearly-stageembryo.Humanembryosreachtheblastocyststage4–5dayspostfertilization,atwhichtimetheyconsistof50–150cellsTheCharacteristicsOfEmbryonicStemCellESCaredistinguishedbytwodistinctiveproperties:theirabilitytoreplicateindefinitelytheirpluripotencyabletodi

6、fferentiateintoallderivativesofthethreeprimarygermlayers:ectoderm,endoderm,andmesoderm.Theseincludeeachofthemorethan220celltypesintheadultbody.TheCharacteristicsOfEmbryonicStemCellMorphologicallyumlimitedproliferationinvitroandmaimtenaceofnormaldiploidkaryotypeHightelomeraseactivati

7、on(telomere)MolecularmarkeralkalinephosphataseOct-4（octamer-bindingtranscriptionfactor4）SSEAs(Stage-SpecificEmbryonicantigens)invitrodifferntiationpotentialintocellsofthreegermlayersCapacitytoformteratoma,wheninjectedsubcutaneoulyintoImmune-deficientmiceInnercellmassasanicheEScellsc

8、anmaketumorsifinwro