小儿哮喘花剥苔产生免疫机制探究

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1、小儿哮喘花剥苔产生免疫机制探究作者：李福凤，李果刚，吴友忠，李靖，章晓鹰，王惠芳，王忆勤【关键词】花剥苔；［摘要］目的：从免疫学角度探讨哮喘患儿花剥苔形成的机制。方法：采用免疫组化方法检测舌苔脱落上皮细胞中角蛋白13及bcl2蛋白的含量。应用流式细胞术检测外周血CD4+及细胞趋化因子受体3(cellchemokinereceptor3,CCR3)的表达量。采用放射免疫法检测血清中皮质醇的含量。结果：哮喘花剥苔组患儿外周血CD4+和CCR3水平明显高于哮喘非花剥苔组及正常组(PV0.05)；哮喘花剥苔组、非哮喘花剥苔组血清中皮质醇含

2、量低于其余各组(P<0.05)o哮喘花剥苔、非花剥苔组血清IgE水平明显高于其余各组(P<0.05)o哮喘花剥苔组、非哮喘花剥苔组舌苔上皮脱落细胞中角蛋白13的含量明显低于哮喘非花剥苔组、非哮喘非花剥苔组和正常对照组(P<0.05)o哮喘花剥苔组患儿舌苔上皮脱落细胞中bcl2蛋白含量与其余各组比较无统计学差异。结论：花剥苔的形成与免疫反应密切相关，血清皮质醇水平降低、CD4+及CCR3增多，可能促进了花剥苔的形成。同时，舌上皮细胞角化障碍及舌上皮细胞凋亡亦可能促进花剥苔的形成。［关键词］花剥苔；哮喘；免疫Immunological

3、mechanismofexfoliativetonguefurinchildrenwithasthmaABSTRACTObjective：Toexploretheimmunologicalmechanismofexfoliativetonguefurinchildrenwithasthma.Methods：Thirtyninechildrenwithasthma,twentyeightchildrenwithrepetitiverespiratorytractinfection(nonasthma)andelevenhealthy

4、childrenweredividedintofivegroups,whichwereasthmawithexfoliativefurorwithnonexfoliativefurgroups,nonasthmawithexfoliativefurorwithnonexfoliativefurgroupsandnormalcontrolgroup.Theconcentra/tionsofkeratin13andbcl2incellsexfoliatedfromtonguefurweredetectedbyimmunohistoch

5、emicalmethod・Theexpressionlevelsofbloodcellchemokinereceptor3(CCR3)andCD4+wereexaminedbyflowcytometry,andthelevelsofserumcortisolandIgEweredetectedbyradioimmunoassay.Results：ThelevelsofbloodCD4+andCCR3ofchildrenwithasthmaandexfoliativefurwerehigherthanthoseintheasthma

6、withnonexfoliativefurgroupandthenormalcontrolgroup(P<0.05).Theserumlevelofcortisolinthegroupsofasthmawithexfoliativefurandnonasthmawithexfoliativefurwerelowerthanthatintheothergroups(P<；0.05).TheserumlevelsofIgEinasthmawithexfoliativefurorwithnonexfoliativefurgroups

7、werehigherthanthatintheothergroups(P&It；0.05).Concentrationsofkeratin13inthecellsexfoliatedfromtonguefurinthegroupsofasthma.ornonasthmawithexfoliativefurwerelowerthanthatoftheothergroups(P<；0.05).Therewasnosignificantdifferenceofexpressionlevelofbcl2inthecellsexfoli

8、atedfromtonguefuramongthesefivegroups・Conclusion：Thereisareasonablycloserelationshipbetweentheformationofexfoliativetonguefu