血清Dickkopf―1蛋白联合CA199检测在胃癌诊断中的应用价值

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1、血清Dickkopf—1蛋白联合CA199检测在胃癌诊断中的应用价值［摘要］目的探讨血清Dickkopf-l(DKK-1)蛋白联合CA199检测在胃癌诊断中的应用价值。方法选取2013年5月〜2014年12月本院收治的58例原发性胃癌患者作为胃癌组，同期收治的60例慢性浅表性胃炎患者作为慢性胃炎组，同时收集60例体检健康人作为健康对照组。3组同时应用酶联免疫吸附法测定DKK-1,应用化学发光法测定CA199,并比较DKK-l、CA199诊断胃癌的特异度、敏感度、准确度、阳性似然比、阴性似然比。结果与慢性胃炎组和健康对照组相比，胃癌组血清DKK-l、CA199水平显著升高

2、(P<0.01)；与单独应用DKK-1或CA199检测相比，DKK-1联合CA199检测的敏感度、准确度、阳性似然比明显升高(P〈0・05),阴性似然比明显下降(P<0.05)o结论血清DKK-1可以作为胃癌临床辅助诊断的依据之一，血清DKK-1联合CA199检测对提高胃癌的诊断水平有独特的优势，值得临床推广应用。［关键词］Dickkopf-l蛋白；胃癌；CA199［中图分类号］R735.2［文献标识码］A［文章编号］1674-4721(2015)07(c)-0121-03ApplicationvalueofserumDickkopf-lproteincombinedw

3、ithCAI99testinthediagnosisofgastriccancerYANQingClinicalLaboratory,JiningTumorHospitalofJiningCityinShandongProvince,Jining272000,China[Abstract]ObjectiveToexploretheapplicationvalueofserumDickkopf-1(DKK-1)proteincombinedwithCAI99testinthediagnosisofgastriccancer・Methods58patientswithpri

4、marygastriccanceradmittedintoourhospitalfromMay2013toDecember2014wereselectedasgastriccancergroup,60patientswithchronicsuperficialgastritisinthesametimewereselectedaschronicgastritisgroup,and60casesofmedicalhealthypeoplewereseiectedashealthycontrolgroup・Inthe3groups,ELISAwasappliedtotest

5、DKK-1andchemiluminesceneewasappliedtotestCA199.Thespecificity,sensitivity,accuracy,positivelikelihoodratioandnegativelikelihoodratioofDKK-1andCAI99wascompared・ResultsComparedwithchronicgastritisgroupandhealthycontrolgroup,levelsofserumDKK-1andCAI99inpgastriccancergroupsignificantlyimprov

6、ed(P<0.01).ComparedwiththesingleapplicationofDKK-1orCA199,thesensitivity,accuracyandpositivelikelihoodratioofDKK-1combinedwithCA199significantlyincreased(P<0.05),andthenegative1ikelihoodratiosignificantlydecreased(P<0.05)・ConclusionSerumDKK-1canbeusedasabasisforclinicaldiagnosisofgastric

7、cancer.SerumDKK-1combinedwithCAI99testdetectionhasauniqueadvantageinthediagnosisofgastriccancer,itisworthyofclinicapplication.[Keywords]Dickkopf-1protein；Gastriccancer；CAI99胃癌是我国常见的消化道恶性肿瘤之…，每年新发病例40多万，死亡20多万，发病率和死亡率远高于世界平均水平[1]。胃癌早期临床表现大多缺乏特异性，因此，对肿瘤发病机制的理解和早期诊断尤为重要。在肿瘤的