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时间:2019-11-26
《缬草挥发油的提取及抗氧化能力研究_李岗》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2015VOL.26NO.11时珍国医国药2015年第26卷第11期缬草挥发油的提取及抗氧化能力研究1,21,21*11李岗,范文垒,余德顺,杨军,田弋夫(1.中国科学院地球化学研究所,贵州贵阳550002;2.贵州大学化学与化工学院,贵州贵阳550025)摘要:应用超临界CO2萃取技术,采用正交实验法,对影响缬草挥发油萃取率大小的萃取压力、萃取温度和CO2流量3个因素进行了研究,应用ABTS和FRAP法对缬草超临界CO2提取物的抗氧化能力进行检测,并与水蒸汽蒸馏法得到的缬草挥发油抗氧化能力
2、进行了对比,用紫外分光光度法对超临界CO2萃取及水蒸汽法得到的缬草挥发油中总缬草三酯进行了测定。结果表明:超临界CO2萃取最佳工艺条件为:萃取压力25MPa,CO2流量20L/h,萃取温度55℃,此工艺条件下的缬草挥发油的收率为5.86%,而水蒸汽法收率为1.27%;ABTS和FRAP法下,缬草超临界CO2提取物之间的抗氧化能力差异不显著,但均强于水蒸汽法且差异显著,超临界CO2萃取法得到缬草挥发油中总缬草三酯含量为3.7%,大于水蒸汽法的2.8%,显示超临界CO2萃取法比水蒸汽法更能有效保留缬草挥发油的生物活性成分及生物活性功能。关键词:缬草挥发油;超临界CO2萃取;水
3、蒸汽蒸馏;抗氧化;ABTS法;FRAP法;总缬草三酯DOI标识:doi:10.3969/j.issn.1008-0805.2015.11.030中图分类号:R284.2;R284.1文献标识码:A文章编号:1008-0805(2015)11-2647-03StudyontheExtractionforEssentialOilofValerianaofficinalisL.anditsAntioxidantCapacity1,21,21*11LIGang,FANWen-Lei,YUDe-shun,YANGJun,TIANYi-fu(1.TheStateKeyofEnviro
4、nmentalGeochemistry,InstituteofGeochemistry,ChineseAcademyofSciences,Guiy-ang,Guizhou,550002,China;2.SchoolofChemistryandChemicalEngineering,GuizhouUniversity,Guiy-ang,Guizhou,550025,China)Abstract:TheessentialoilsofValerianaofficinalisL.wereobtainedwithsupercriticalCO.Byusingthemethodofo
5、rthogonal2experiment,theextractionpressure,extractiontemperatureandCO2flowratewerestudied.Theantioxidantcapacitiesofthesees-sentialoilsweredetectedwithABTSandFRAPmethods,andthecomparionweremadewiththatoftheessentialoilextractedbystreamdistillation.TotalvalepotriatesweredetectedbyUVSpectro
6、photometrybothfortheessentialoilsextractedbysupercriticalCO2andstreamdistillation.Theresultsshowthat:ForsupercriticalCO2extraction,theoptimizationextractionpressurewas25MPa,temperaturewas55℃,andCO2flowratewas20L/h.Intheseprocessconditions,theextractionrateofessentialoilwas5.86%.Theextract
7、ionrateofessentialoilextractedbystreamdistillationwas1.27%.BothforABTSandFRAPmethods,theantioxidantcapacitiesamongtheseessentialoilsextractedbysupercriticalCO2processwerenosignificantdifference.Butthean-tioxidantcapacitiesbetweentheessentialoilsextractedbysupercriti
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