临床医学论文-儿童急性淋巴细胞白血病TELAML1融合基因的表达水平与临床特点、早期治疗

《临床医学论文-儿童急性淋巴细胞白血病TELAML1融合基因的表达水平与临床特点、早期治疗》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、临床医学论文•儿童急性淋巴细胞白血病TELAML1融合基因的表达水平与临床特点、早期治疗反应的关系作者：李志刚，赵玮，高超，吴散媛，张永红，石慧文，谢静【摘要】为探讨儿童急性淋巴细胞白血病初诊时TELAML1融合基因表达水平与患儿的临床特点、早期治疗反应的关系，采用实吋定量聚合酶链反应（RQPCR）定量检测35例患儿（包括标危20例，小危15例）初诊TELAML1表达水平和诱导缓解治疗结束微小残留病（MRD）水平，比较MRD阴性与MRD阳性患儿的初诊TELAML1表达水平及临床特点，分析初诊TELAML1表

2、达水平、MRD水平与临床特点、早期治疗反应的相关性。结果发现，初诊时TELAML1表达水平为1.63X104拷贝/104拷贝ABL（中位数）。诱导缓解结束时，16例（10例标危、6例中危）患儿未达到分子缓解,MRD水平分别为0.84-282.93拷贝/104拷贝ABLo初诊TELAML1表达水平与各临床特点及MRD水平缺乏相关性。MRD水平与泼尼松实验治疗第8天外周血幼稚细胞数显着相关。初诊时外周血白细胞计数V25X109/L的患儿，MRD水平还与白细胞计数、幼稚细胞百分比显着相关。MRD阴性患儿初诊TEL

3、AML1表达水平显着低于MRD阳性者。结论:对于TELAML1+儿童ALL,45.71%未能在诱导缓解治疗结束时获得分子缓解，说明了后续治疗的重要性。泼尼松实验治疗的效果预示了诱导缓解治疗结束时的MRD水平。初诊外周血白细胞计数、幼稚细胞百分比和初诊TELAML1表达水平均在一定程度上影响诱导缓解治疗结束时的MRD水平。【关键词】急性淋巴细胞白血病实吋定量PCRTELAML1微小残留病RelationbetweenTELAML1ExpressionLevelandClinicalCharacteristic

4、saswe11asEarlyResponsetoTreatmentinChildrenwithAcuteLymphoblasticLeukemiaAbstractThestudywasaimedtoinvestigatetherelationbetweentheexpressionlevelofTELAML1(translocationETSleukemiaacutemyeloidleukemia1)fusiongeneandclinicalcharacteristicsaswellasearlyrespo

5、nsetotreatmentinchildrenwithALL(acutelymphoblasticleukemia)・Withrealtimequantitativepolymerasechainreaction(RQPCR),theexpressionlevelofTELAML1atdiagnosisandMRD(minimalresidualdisease)attheendofinductionofremissionweredetectedin35childrenwithALL,including20

6、SR(standardrisk)and15IR(intermediaterisk)patients・TheexpressionlevelofTELAML1andclinicalcharacteristicsatdiagnosiswerecomparedbetweenMRDnegativeandMRDpositivepatients・TherelationbetweenTELAML1expressionlevelsatdiagnosis,MRDlevelandclinicalcharacteristicsas

7、wellasearlyresponsetotreatmentwerealsoexplored.TheresultsindicatedthattheexpressionlevelsofTELAML1atdiagnosiswere1.63X104copics/104copiesABL(median)・Attheendofinductionofremission,16patients(10SRand6IRpatients)didnotachievemolecularremission,whoseMRDlevels

8、were0.84-282.93copies/104copiesABL.NorelationwasfoundbetweenexpressionlevelsofTELAML1atdiagnosisandclinicalcharacteristicsaswellasMRDleve1.TherewasasignificantrelationbetweenMRDlevelandblastcountinperipheralb