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1、简易液基涂片技术在痰液检查中的运用［摘耍］冃的：比鮫简易液基涂片技术与传统涂片技术在痰液细胞学的制片效果及痰癌细胞的诊断阳性率。方法：收集患者清晨咳嗽示的痰液置于封闭赠料瓶屮，分为两组,一组做传统涂片，另一组用简易液基涂片方法制片。光镜下观察比较两者的制片质量并评估优劣。结果：110例标本中，临床最后诊断42例(38.18%)为阳性,68例(61.82%)为阴性。简易液基涂片中找到癌细胞34例(30.91%),可疑癌细胞5例(4.54%)中除1例临床诊断为阴性要求随访外都为阳性患者;传统涂片中找到癌细胞20例(18.18%),可疑癌细胞14例(12.
2、73%),其屮5例液基涂片诊断同为可疑，另7例临床为阳性患者，另2例临床诊断为阴性需要随访。结论：简易液基涂片技术较传统涂片技术能冇效地提高痰液细胞学制片质量及痰癌细胞诊断的阳性率。［关键词］液基涂片技术；传统涂片；痰液；肺癌［中图分类号］R734［文献标识码］A［文章编号］1673-7210(2011)04(a)-048-02Applicationofsimple1iquidbasedsmearinsputumexaminationCHENRongl,JIANGChunfan21.DepartmentofPathology,WuhanMedical
3、TreatmentCenter,Wuhan430023,China;2・DepartmentofPathology,theCentralHospitalofXiangfanCity,Xiangfan441021,China［Abstract］Objective:Tocomparethepositiveratioofsimpleliquidbasedsmearandtraditionalsmearinsputumcytology.Methods:Thedeeplyinsidesputumofpatientsinthemorningwerecollect
4、ed,andweredividedintotwogroups.Onewasdonebythetraditionalcytology,theotherwasdonebysimpleliquidbasedsmear.Theresultsunderthemicroscopeandthequalitiesofbothslideswerecomparedtoevaluatewhichwasbest.Results:110casesofsamplesinthefinallyclinicaldiagnosis,42cases(38.18%)forpositive,
5、68cases(61.82%)fornegative.Simpleliquidbasesmearfoundin34patients(cancercells,30.91%),suspiciouscancercellsin5patients(4・54%)inallthecasesclinicaldiagnosisfornegativerequirementsfollow-upoutsideal1positiveforpatients;traditionalsmearfoundin20patients(18.18%)cancercells,suspicio
6、usof14cases(12.73%),ofwhich5caseswerediagnosedwith1iquidbasesmearassuspicious,theother7casesclinicaltestedpositiveforthepatient,another2casesclinicaldiagnosisfornegativeneedfollow-up・Conclusion:Simpleliquidbasedsmearismuchbetterthantraditionalsmearinsputumcytologyslidequalityan
7、dpositiveratioofcareinomacells.［Keywords］Liquidbasesmeartechnology;Traditionalsmear;Sputum;Lungcancer肺癌在我国的发病率及死亡率一直很高，痰液中查找癌细胞无论是对肺癌的筛查述是诊断都是简易且无创伤的有效手段之一。在宫颈癌前病变方而，液基薄层技术较传统刮片检杳能大大提高阳性率,但是对于痰液检查,新柏氏TCT等液基薄层制片因其制作过程繁琐且成本较高，限制了其广泛应用。本实验将110例痰液先传统涂片后再用简易液基涂片，比较其制片效果及诊断结果。1材料与方法1
8、.1材料选取我院（武汉市医疗救治中心）2009年6月〜2010年5月因肺部阴影待杏的住院患者送检痰标本110
