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1、带格式的:字体:ArialNarrow,小三带格式的:居中带格式的〔带格式的[・・・「(带格式的m(带格式的:£带格式的〔…〕f带格式的J带格式的:顶端:2.5网米,底端:2.5厘米[带格生的CTL带格多的匚二带格式的:字体:ArialNarrow「带格式的■厂‘IdentificationandAnalysisofTn5TransDOSonTransposed.InsertsMSitesin・AvirulentMutantsofRalstoniasolanacearum▲▲CheJiarvmeii,MaGui・mei2,LiuBQ—ZhuYu
2、・iingi,ZhengXue・fanq!,TangJiarvvanT丄(1.AgriculturalBio・ResourceResearchInstitute,FujianAcademyofAgriculturalSciences,FujianFuzhou,350003,=Chinq;2.KevLaboratoryofBiopesticideandChemicalBiologyFuiianAgricultureandForest”UniversityFuzhou350002,China)丄Abstract:MulanisofRais伽iaso
3、kmacwrumstrainFJAP91usingTn5iransposonmulagenesisweregolin(hisDaper,whichwerenamedFJAT・t582andFJAT・t583・J?a/s伽bacterialwillToobtainavielentnu4ansinqEZ・Tn5并ansposonrandominsertRsobn/eBivmandfese审chin^theinsertsitesandggIgrv円o申holog米o口he4wo~sgB*viealeAJffurtaftfsColonymorpho
4、logyandattenuationindexesshowedthatbothofthemutantsFJAT・t582andFJAT・t583wereavirule"strains.Andattenuationindexesofthese2strainswereover0.8.A681bpfragmentoftheRanamycinresistancegenqwasamplifiedfromthegenomeDNAofthemutantstrains^Jheinsertionsitesofthese2avirulentmutantswerqJ
5、ocated讥4
6、Tesulte—Ihat-tfansposoR-iRseFt—ift~putativediguanylatephosphodiesterasegeneandtypeJll.effectorproteingene,respectively-^.ItwassuggestedthatmutationsofAheseIhese2genes厂nurtatien-wouldaffectthepathogenicityoftheRsolanacearumstrainsjeadtosto佛a曲%enita护的洋伽間€巒ifulen好ealtH
7、QKeywo〔d、Ralstoniasolanacearuni^virulentMutentmutanl曲enuationAttenuationindex般式的:字体:Aria.Narrow,[带格迭的[・I带格丢的:居中带格式的:字体:ArialNarrow,四号带格式的带格式的:字体:(国际)ArialNarrow[带格式的:屈中[带格式的,带格式的「带格式的匚二▲青枯雷尔氏菌是引起植物细菌性枯萎病的病原菌,广泛分布丁•热带、亚热带和温带,其寄主范I带格式的:字体:ArialMacow堆金项II:“H农村领域国家科技汁划课題(NO.2012
8、AA101504),国家星火计划项丨
9、(No.S20110410006)和福建省财政”项•福建•省农业科学院科技创新团队建设加金(No・STlF・Y03)作者简介:乍加(1977・),女,山东省乳山帀人,副研究閔,叫」:,研究方向为生物技术与生物防治°Tch0591・87882571:E・m“ihchcim2002@163・com。拿通讯作者:刘波《1957・).男.福建省惠安具人,研究员,博上,研究方向为微工物住物技术与农业生物药物。Ibh13905917339:E・maih』iubofaas@[63.com:彎式的:行距:多倍行距L25I域
10、代码豆更改带格式的:術萄牙语(巴西)~青枯雷尔氏菌卩5,转座子无致病力突变株插入位点的鉴定与分析车建美I,马桂美2,刘波「,朱育菁1,郑雪芳I,唐建阳