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时间:2018-04-16
《番茄青枯雷尔氏菌强致病力菌株变异的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、广西植物GuihaiaJul.2014,34(4):530-534http://journal.gxzw.gxib.cnDOI:10.3969/j.issn.1000-3142.2014.04.020马健尧,李焕秀,李立佼,等.番茄青枯雷尔氏菌强致病力菌株变异的研究[J].广西植物,2014,34(4):530-534MaJY,LiHX,LiLJ,etal.VariationofvirulentstrainsofRalstoniasolanacearumisolatedfromtomato[J].Guihaia,2014,34(4):530-
2、534番茄青枯雷尔氏菌强致病力菌株变异的研究马健尧,李焕秀*,李立佼,谭华强,张莉,铁曼曼(四川农业大学园艺学院,四川雅安625014)摘要:为研究番茄青枯雷尔氏菌强致病力菌株的变异,探索了继代培养、在NB培养基上不同时间培养、不同pH处理7d和15d、不同温度处理1h后对强致病力菌株变异的影响。结果表明:随着继代培养的培养代数增加,平均弱化指数成增大趋势,在第10代出现了无致病力菌株;在NB培养基上培养15d时,强致病力菌株已完全转化为不确定菌株和无致病力菌株,在培养30d时,强致病力菌株几乎完全转化为无致病菌株;pH7.0时,处理7d和1
3、5d后,强致病力菌株比例均为最大,分别为93.33%和92.22%,pH5.8时,强致病力菌株比例最低,分别为46.67%和31.11%;用不同温度处理强致病力菌株发现,温度50℃时,菌株死亡,温度40℃时,活菌数显著低于其他(4~30℃)处理,强致病力菌株比例为4~40℃所有处理中最低。关键词:青枯雷尔氏菌;强致病力菌株;无致病力菌株;弱化指数中图分类号:S436文献标识码:A文章编号:1000-3142(2014)04-0530-05VariationofvirulentstrainsofRalstoniasolanacearumisol
4、atedfromtomato*,LILi-Jiao,TANHua-Qiang,MAJian-Yao,LIHuan-XiuZHANGLi,TIEMan-Man(CollegeofHorticulture,SichuanAgriculturalUniversity,Ya’an625014,China)Abstract:InordertostudythevariationofRalstoniasolanacearumvirulentstrainsisolatedfromtomato,theinfluenceofsubculturetimes,dif
5、ferentculturetimeonNBmedium,differentpHin7dand15dtreatments,1htreatmentatdifferenttemperaturesonthevariationofvirulentstrainswereinvestigated.Theresultsshowedthattheaverageattenuationindexraisedwiththeincreasingofsubculturegeneration,andavirulentstrainappearedinthetenthgene
6、ration.Virulentstrainshadbeencompletelytransformedtouncertainvirulentstrainsandavirulentstrainsafter15donNBmedium,andthenal-mostbecameavirulentstrainsafter30d.Virulentstrainsaccountedforthelargestproportionafter7dand15dcultiva-tionunderpH7.0,therateswere93.33%,92.22%respect
7、ively.Onthecontrary,virulentstrainsoccupiedtheleastpro-portionunderpH5.8withthepercentageof46.67%,31.1%respectively.Whenvirulentstrainsweretreatedbythedif-ferenttemperatures,thestrainsdeadat50℃.Andwhenthetemperaturewas40℃,thenumberoflivestrainswassig-nificantlylowerandthevi
8、rulentstrainswereatlowerratiothananyothertemperature(4-30℃).Keywords:Ralstoniasola
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