生防菌ANTI8098A对青枯雷尔氏菌不同致病力菌株抑制作用的差异性

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1、生防菌ANTI-8098A对青枯雷尔氏菌不同致病力菌株抑制作用的差异性葛慈斌林抗美朱育菁苏明星刘波*（福建省农业科学院生物技术研究所生物农药研究中心，福建福州350003）摘要：采用共培养法测定了生防菌ANTI-8098A对青枯雷尔氏菌强致病力菌株F-01-V和无致病力菌株F.1.3-010602-01-A的抑制作用，并以链霉素作参照。结果表明，生防菌ANTI-8098A对F-01-V的抑制作用较强，抑制率与生防菌培养液菌体数成正相关；对F.1.3-010602-01-A的抑制作用则较弱，菌体数为20.0×108cfu•mL-1的生防菌液对F.1.3-010602-01

2、-A的抑制率为14.7%，而对F-01-V的抑制率高达99.5%。链霉素对F-01-V和F.1.3-010602-01-A都具有较强的抑制作用，抑制率与浓度成正相关，但是对F.1.3-010602-01-A的抑制作用更强：浓度为0.25U•mL-1的链霉素溶液，对F.1.3-010602-01-A的抑制率为50.0%，而对F-01-V的抑制率仅为3.3%。应用生防菌ANTI-8098制剂进行田间防治茄子青枯病的试验结果表明，生防菌制剂的防治效果达92.11%，显著地比农用链霉素的防效（72.81%）高。研究结果为阐述生防菌ANTI-8098对青枯雷尔氏菌的致弱现象提供了

3、依据。关键词：生防菌ANTI-8098A；链霉素；青枯雷尔氏菌；抑制作用中图分类号：文献标识码：AStudyontheDifferencesbetweentheInhibitionsofBiocontrolBacteriumStrainANTI-8098A(Bacilluscereus)againstRalstoniasolanacearum-VirulentandAvirulentStrainsGECi-bin，LINKang-mei，ZHUYu-jing，SUMing-xing，LIUBo*（BiocideCenter，InstituteofBiotechnolog

4、y,FujianAcademyofAgriculturalScience，Fuzhou350003，China）Abstract:TheinhibitionsofthebiocontrolbacteriumstrainANTI-8098A(Bacilluscereus)againstRalstoniasolanacearumvirulentstrainF-01-VandavirulentstrainF.1.3-010602-01-Awereinvestigatedandstreptomycinwasusedascontrol.Theresultsshowedthatth

5、einhibitionofstrainANTI-8098AagainststrainF-01-Vwasstrong.TherewasapositivecorrelationbetweentheinhibitingrateandthebacterianumberofstrainANTI-8098A.TheinhibitionofstrainANTI-8098AagainststrainF.1.3-010602-01-Awasweak.TheinhibitingratesofthecultureofstrainANTI-8098Awiththebacterianumber2

6、0.0×108cfu•mL-1againststrainF-01-VandstrainF.1.3-010602-01-Awere99.5%and14.7%,respectively.StreptomycincouldinhibitthegrowthofstrainsF-01-VandF.1.3-010602-01-A.Therewasapositivecorrelationbetweentheinhibitingrateandtheconcentrationofstreptomycin.Buttheinhibitionofstreptomycinagainststrai

7、nF.1.3-010602-01-AwasgreaterthantheinhibitionagainststrainF-01-V.Theinhibitingratesofstreptomycinwiththeconcentrationof0.25U•mL-1againststrainF-01-VandstrainF.1.3-010602-01-Awere3.3%and50.0%,respectively.Thefieldexperimentoncontrollingthebacterialwiltdiseaseofeggplantindi