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时间:2019-10-30
《精子质量与形态学分析及其关系》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、2248实用医学杂志2009年第25卷第14期精子质量与形态学分析及其关系周其赵陈思梅冯春琼邹亚光孙德华李铁求李飞毛向明摘要目的:探讨精子质量与形态学的临床应用价值及其关系。方法:248份精液标本按WHO标准进行精液常规分析,应用计算机辅助精液分析(CASA)分析精子密度、活力、活率以及动态参数;采用WHO推荐的Diff-Quik快速染色方法对精子形态进行染色;采用SPSS13.0软件进行统计学分析。结果:248份精子质量分析中,精子活力不足占74.6%,精子活力正常占25.4%,少精症占5.6%,少弱精症占4.8%,正常精液占24.
2、6%;248份精子形态学分析中,形态正常精液占73.4%,畸形精子症占26.6%。精子活力和活率与形态正常精子百分率呈显著性正相关(r=0.52,r=0.53,P<0.01);各运动参数和精子密度也与形态正常精子百分率呈显著性正相关(P<0.01)。精子活力正常组形态正常精子百分率明显高于精子活力低下组(P<0.01)。结论:精子活力不足和畸形精子症是导致男性不育的主要原因,精子形态与精子活力、活率、密度和运动参数有密切关系,精子质量和形态学分析在临床男性不育的诊断中具有重要作用。关键词不育,男(雄)性;精子形态;精子活力;精子密度;
3、年龄AnalysisofspermqualityandmorphologyZHOUQi-zhao*,CHENSi-mei,FENGChun-qiong,ZOUYa-guang,SUNDe-hua,LITei-qiu,LIFei,MAOXiang-ming.*DepartmentofUrology,NanfangHospital,SouthernMedicalUniversity,Guangzhou510515,ChinaCorrespondinganther:MaoXiang-mingE-mail:mxm@fimmu.com【Abst
4、ract】ObjectiveToinvestigatetheclinicalvalueofspermqualityandmorphologyandtheirassociation.Methods248semensampleswereexaminedaccordingtotheWHOcriteria.Thespermdensity,motility,viability,anddynamicparameterswereanalyzedbycomputer-aidedspermanalysis(CASA);spermmorphologywa
5、sdetectedusingDiff-QuikstainingrecommendedbyWHO.ThesoftwareSPSS13.0wasusedforstatisticalanalysis.ResultsPoorspermmotilitywasfoundin74.6%ofallthesemensamples,normalmotilityin25.4%,Oligospermiain5.6%,asthenospermiain4.8%,andnormalsemenin24.6%.Thespermmorphologywasnormalin
6、73.4%ofthesamplesandteratozoospermiaoccurredin26.6%.Spermmotilityandviabilitywerepositivelyrelatedwiththepercetageofnormalmorphology(r=0.52,r=0.53,P<0.01),sowereeachdynamicparameterandspermdensity(P<0.01).Thepercentageofnormalspermmorphologywasgreaterinthenormalmotility
7、groupthanintheasthenospermiagroup(P<0.01).ConclusionsAsthenospermiaandteratozoospermiaarethemajorfactorsformaleinfertility.Thespermmorphologyiscloselyrelatedtospermmotility,viability,densityanddynamicparameters.Theanalysisofspermqualityandmorphologyplaysanimportantrolei
8、ntheclinicaldiagnosisofmaleinfertility.【Keywords】Infertility,male;Spermmorphology;Spermmotility;Spermdensity;A
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