肺癌患者外周血中MDR1的表达及临床意义

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1、肺癌患者外周血中MDR1的表达及临床意义左翠云，孙志强，吴平，周志忠，黎喜梅，胡耀斌，吴建伟【摘要】目的检测多药耐药基因・1(MDR1)在肺癌患者外周血中的表达，探讨MDR1的表达与肺癌病理类型及临床化疗疗效、肺癌耐药性之间的关系。方法应用荧光定量PCR(qRT-PCR)技术，动态监测45例肺癌患者化疗前后外周血中MDR1的表达水平，并与48例健康者进行对照比较。结果肺癌组化疗前MDR1的阳性率28.89%(13/45),明显高于健康对照组2.08%(1/48)(P<0.05)；各种类型的肺癌患者随着化疗次数的增多MDR1的表达均增强；化疗

2、前后肺鳞状细胞癌和肺腺癌MDR1的表达明显高于小细胞肺癌(PvO.05)。结论肺癌患者外周血中MDR1表达水平与接受化学药物治疗密切相关，化疗可诱导各种病理类型肺癌MDR1表达增加；非小细胞肺癌(NSCLC)及小细胞肺癌(SCLC)在化疗耐药机制上可能存在差异:NSCLC可能为原发性MDR,SCLC可能为获得性MDR；临床上可根据MDR1表达情况合理制定化疗方案。【关键词】肺癌；多药耐药基因外周血；荧光定量PCRExpressionandclinicalsignificanceofmultidrugresistancegeneMDR1inp

3、eripheralbloodofpatientswithlungcancerZUOCui-yun,SUNZhi-qiang,WUPing,ZHOUZhi-zhong,LIXi-mei,HUYao-bin,WUJian-wei.TheDepartmentofRespiratoryDisease,TheThirdHospitalofXiaCity,Xiamen361100,Fujianprovince,China【Abstract1ObjectiveThisstudywasdesignedtoquantitativeanalysisofmult

4、idmgresistancegene(MDR1)levelsinperipheralbloodanddiscusstheimpactofMDR1levelsuponpathologicaltypesoflungcarcinomaandclinicalchemotherapyresponse、Lungcancerdrugresistance.MethodsFluorescencequantitativereversetranscriptionpolymerasechainreaction(qRT-PCR)wasusedtoexaminethe

5、expressionofMDR1levelsinperipheralbloodof48healthypersonsand45lungcancercases・Results13cases(28・89%)werepositiveforMDR1expressioninlungcancersand1cases(2.08%)inhealthypersonsbeforechemotherapy,therewasastatisticallysignificantcorrelation(P<0.05).TheexpressingintensityofMDR

6、1wasenhancedbythetreatofchemotherapyinpathologicaltypesinlungcancer.TheexpressionofMDR1levelswassignificantlyhigher(P<0.05)inadenocarcinomaandsquamouscellcarcinomaoflungcancerthanthatinsmallcelllungcance匚ConclusionsThemRNAlevelsofMDR1expressionintheperipheralbloodoflungcan

7、cerpatientsiscloselyreleatedtochemotherapy,ChemotherapymayincreasetheincidecerateofMDR1expressioninlungcancer；theremaybedifferenceofchemotherapyresistancemechanismbetweenNSCLC(nonsmallcelllungcancer)andSCLC(smallcelllungcancer)：NSCLCaremainlyintrinsicMDRwhileSCLCismainlyac

8、quiredMDRtochemotherapy.ThetreatmentplancouldbereasonablymadeaccordingtotheexpressionofMD