膏桐快繁技术研究

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1、西南林学院硕士学位论文膏桐快繁技术研究姓名：唐军荣申请学位级别：硕士专业：森林培育指导教师：马焕成200805011・不定芽诱导及快繁途径，采川健壮的膏桐种子作为初始外植体，洒精消毒30s,升汞7min,有效外植体数达100%,然后取种胚在MS培养基上获得无菌苗，一个星期后取无菌苗的叶片和茎段，接种于MS+6-BAO.5mg/L+KTO.5mg/L+NAA枭基¥金?通人缶严夢5d后便可产生不定芽，最后将不定芽切割，转接到MS+6—BAO.5mg/L+KTO.5mg/L+NAAO.02mg/L的培养基上进行增殖，其有效增殖率可以达到3.8倍，最后在MS+NAAO.2mg/L+1

2、59/L蔗糖+29/L碳粉的生根培帝基上诱导生2•腋芽诱导及快繁途径，采取当年生未木质化的嫩枝，酒精30S,升汞消毒1imin,然+,s,s后M有效外植体数可达70%,在MS+6-BA1.Omg/L+NAAO.lmg/L诱导出腋芽6-BA1.0mg/L+NAhO.02mg/L上进行增殖培养，其平均增殖倍数3・2后存NAAO.2+159/L蔗糖+29/L碳粉的生根培养基上诱导生欝于生根苗在自然光照条件下过渡7d,洗净培养基后，川低浓度的多菌灵浸泡2min,栽种于消过毒的河沙基质屮，塑料棚保湿，每隔3-5d，川多菌灵喷洒一次,并浇灌1/2MS溶液，10d后可逐渐移除塑料膜，30d

3、后可移栽到大田中，其成活率可以达80%以上。关键词：膏桐，抨插繁殖，组织培养，生根，炼苗移栽yEuPh0rb•acea,wh•1chd•1str•1but1ydstrbutesdry—h0tVa11ey•res•stanttr0p•eswidelyintropicannsouthwesternChinca1landsubtropicaa.Inthelastfew1regions,andmainyears,asadroughttrccuscdtogrowinAmericaandthctrridzoncofAsia,foritsstrongcradaptabi1itytothcc

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