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《甘遂毒性的血浆代谢组学研究杨永霞1，唐冰雯2，丁佳佳2，王琳琳1》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、甘遂毒性的血浆代谢组学研究杨永霞1,唐冰雯2,丁佳佳2,王琳琳】，毋福海3,宋粉云2(510006r州，广东药学院：基础学院】，药科学院2,公共卫生学院3)［摘要］目的研究甘遂毒性致人鼠血浆代谢组的变化，探讨代谢组学在中药毒性研究中的可行性。方法选取SD大鼠18只，适应1周后随机分为3组：对照组、甘遂低剂量组(7.875g/kg)和甘遂高剂量组(15.75g/kg),持续给予甘遂2周后，取各组人鼠血浆，收集各组血浆样品的］HNMR谱，运用正交最小二乘判别(orthogonalpartialleast-squares-discriminantanalysis,O
2、PLS-DA)方法分析。结果与对照组相比，甘遂组血浆代谢有明显变化。甘遂低剂量组血浆中乳酸和肌酸的含量升高，月旨、3-疑丁酸盐、牛磺酸和葡萄糖的含量降低。此外，丙氨酸、0-乙酰糖蛋白和氧化三甲胺在高剂量甘遂组中升高，而不饱和脂肪酸则降低。结论本实验从分子水平阐明了甘遂毒性引起的血浆代谢组变化。［关键词］甘遂；代谢组学；核磁共振氢谱［中图法分类号］［文献标志码］A［基金项目］国家自然科学基金(21005022)；广东省自然科学基金(S2011010002512)［通信作者］杨永霞，电话：(020)39352197,E-mail:sheepma@163.com宋粉
3、云，电话：(020)39352136,E~ma订：fuhaiwu@163.comPlasmametabonomicchangesinducedbyEuphorbiaKansuitreatmentbasedon〔HNMRspectroscopy］22i3212YangYongxia,TangBingwen,DingJiajia,WangLinlin,WuFuhai,SongFenyun(BasicSchool,CollegeofPharmacy,3SchoolofPublicHealth,GuangdongPharmaceuticalUniversity,Gua
4、ngzhou,GuangdongProvinee,510006,China)［Abstract］ObjectiveToclarifytheplasmametabonomicchangesinducedbyKansuitreatmentrevealingthetoxicitymechanismofKansui.Methods18SDratsweredividedintothreegroups,i.e.controls,lowdosageofKansuigroup(7.875g/kg)andhighdosageofKansuigroup(15.75g/kg).Af
5、ter2weeks,plasmasampleswereobtainedand!HNMRspectraofeachsamplewasperformedandanalyzedbyOPLS-DA(orthogonalpartialleast-squares-discriminantanalysis)method.ResultsIncomparisonwiththecontrolgroup,theplasmametabonomicprofilesofKansuitreatmentgroupshavesomeimportantchanges.Lactateandcrea
6、tineincreased,andlipids,3-hydrobutyrate,taurineandglucosedecreasedintwoKansui-treatedgroups.Meanwhile,thelevelsofalanine,O-acetyl-proteinandTMAOhaveanincreaseandunsaturatedfattyaciddecreasedinhighdosageKansuitreatmentgroup.ConclusionKansuiinducedobviousplasmametaboliteschanges.[Keyw
7、ords]Kansui;Metabonomics;nuclearmagneticresonance'HspectroscopySupportedbytheNationalNaturalScienceFoundationofChina(21005022)andtheNaturalScienceFoundationofGuangdongProvince(S2011010002512).Correspondingauthor:YangYongxia,Tel:86-20-39352197,E-mail:sheepma@163.com;SongFenyun,Tel:86
8、-20-39352136,E-mail
