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1、UNIVERSITYOFELECTRONICSCIENCEANDTECHNOLOGYOFCHINAMASTERTHESIS201521210311()1UDC1TheresearchofspectrumanalysisinplasmaH-NMRmetabolomicsAMasterThesisSubmittedtoUniversityofElectronicScienceandTechnologyofChinaDiscipline:BiomedicalEngineeringAuthor:LiuZhanSupervisor:Prof.Jian
2、gHuaSchool:SchoolofMedicine[1][2-5][6][7][8][9][10][11][12][13][14][15]ABSTRACTAbstractMetabolomicsisanewgenerationofomicstechniquesthatcombinecloselywithclinicalresearchanddevelopedrapidlyinrecentyears.Metabolomicshasbeenoneoftheresearchhotspotbecauseofitcouldanalysisthem
3、oleculemetabolitesofcomplicatedbiologicalsamples[1].NuclearMagneticResonance(NMR)andMassspectra(MS)arethetwomostimportantanalysisplatforminmetabolomicsatpresent,butNMRhasbeenwidelyusedbecauseofitssimplesamplepreparation,highprecision,nondestructiveandnon-deviation,allofthe
4、secharactermakeithaveagreatsuperiorityinanalyzingthemetabolitesofbiologicalsample.IntheheadresearchofNMRmetabolomics,Inordertomaximizethesampleavailabilityinformation,researcherhavedonemanyresearchinsamplepreparation,NMRexperimentaldesign,temperature,thesettingofrelaxation
5、time,eventhesuppressionmethodofsolventpeak.AlsofortheNMRspectra,whichhavedevelopedmanyprocessingmethodinordertoconvenientforthenextspectradataprocessing,likecorrectionofphase,baselinecorrection,spectraaligning,normalizationprocessing,integration,deconvolution,quantitativem
6、ethod.Butthebiologicalsamplessuchasplasma,urineandcerebrospinalfluid,therearesomanymolecularmetabolitesthatcausedNMRspectraalotofoverlapinevitable.MetaboliteidentificationaccordingtoanalyzingtheNMRspectraisthekeyofNMRmetabolomicsnextdevelopment,buttheoverlapofspectrapeakis
7、biggestchallengeofNMRmetabolomics.ThetraditionalsolutiontosolvepeakoverlapisexpandtheNMRexperimenttotwo-dimentionalhomonuclear(suchasJRES,COSYandTOCSY)orheterocaryosis(suchasHSQCandHMBC),butthetwo-dimentionalNMRexperimenttimeis10-40timescompareto1H-NMR,therefore,thetwo-dim
8、entionalNMRisnotsuitableforhighthroughputandlargesampleofNMRmetabolomics.Whenintheprocess
