食品中甜味剂的离子对高效液相色谱法测定

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1、食品中甜味剂的离子对高效液相色谱法测定杨小琪黎源倩邹晓莉成都市食品药品检验研究院四川大学华西公共卫生学院（华西第四医院）摘要：目的建立食品中4种甜味剂（乙酰磺胺酸钾、糖精钠、阿斯巴甜和环己基氨基磺酸钠）的离子对高效液相色谱（IP-HPLC）测定方法。方法发酵酸奶样经沉淀剂（K[Fe（CN）2]、Zn（Ac）2）沉淀去除蛋白后提取;榨菜样匀质后用蒸镭水浸提;饮料样离心再用0.45um微孔滤膜过滤后直接上样分析。采用5mmol/L三耗甲基氨基甲烷缓冲溶液-甲醇（95:5,v/v）为流动相，C18色谱柱分离，紫外检测波长205nmo结果

2、食品中待测化合物的质量浓度与峰面积成良好的线性关系，乙酰磺胺酸钾、糖精钠、阿斯巴甜、环己基氨基磺酸钠线性范围分别为0.1360ug/mb0.0/0ug/ml>0.1060ug/mb0.0071.0mg/ml;方法检出限分别为0.040、0.020、0.030、2.Oug/mlo酸奶、榨菜样品中4种组分的平均加标回收率为82.l%l08.6%o相对标准偏差（RSD）为1.16%6.95%□结论离子对高效液相色谱法首次同时对食品中的4种甜味剂进行分析，本法不需衍生，测定快捷、操作简单，用于实际样品的测定取得满意的结果。关键词：甜味剂;

3、食品;离子对-高效液相色谱法;作者简介：杨小琪（1981-），女，主管技师，硕士，研究方向：食品理化收稿日期：2017-04-30Determinationoffoursweeteneradditivesinfoodbyion-pairreversephasehighperformanceliquidchromatographyYANGXiao-qiLIYuan-qianZOUXiao-liChengduInstitutionforFoodandDrugControl;Abstract：ObjectiveToestablished

4、anovelmethodofion-pairreverse-phasehighperformanceliquidchromatography(IPHPLC)forsimultaneousdeterminationoffoursweetenetadditivesineludingacesulfame-K,aspartame,sodiumcyclamateandsodiumsaccharineinfood.MethodsProtoininyoghurtsamplewasprecipitatedwithpotassiumhexacyan

5、oferrateandzincacetateandthenitextracteclbydistilledwater.Preservedpicklewashomogenizedandextractedbydistilledwater.Beveragewasfiltratedwith0.45mfiltermembraneandthenthesweetenetsinal1thesampleswereanalyzedwithhighperformance1iquidchromatography.Thescpcrationwasaccomp

6、lishedonaC18columnwiththemobilephaseof5mmol/Ltries(hydroxymethyl)aminomethaneaqueoussolution(pH4.5,adjustedby4mol/Laceticacid)atflowrateof1.20mL/minandthedetectionwavelengthwas205nm.ResultsThereweregood1inearre1ationshipontheconcentrationsoftheanalyteandtheirpeakareas

7、intherangeof0.13ug/mL-60ug/mLforaccsulfame-K,0.07Pg/mL-60ug/mLforsodiumsaccharine,0・10ug/mL-60ug/mLforaspartameand0.007mg/mL~4.0mg/mLforsodiumcyclamate,respectively.TheirlimitsofdetectionwereO.040Ug/mL,0.020Ug/mL,0.030ug/mL,2.0ug/mL,respectivelyTheaveragerecover!esoft

8、hemethodforyoghourtandbeveragewas82.l%-108.6%withtherclativcstandarddeviations(RSDs)of1.16%-6・95%.ConclusionTheion-pairrever