脑心通胶囊对血管性痴呆模型大鼠的治疗作用（医学论文）

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1、脑心通胶囊对血管性痴呆模型大鼠的治疗作用【关键词】脑心通胶囊；”大脑中动脉闭塞；“血管性痴呆；“学习记忆；，誨马摘要：目的观察脑心通胶囊对血管性痴呆模型大鼠行为学及海马组织形态学的作用。方法采用大脑中动脉闭塞法(MCAO)制作血管性痴呆动物模型；跳台试验测定大鼠学习记忆成绩；取脑组织作冰冻切片，HE染色，观察大鼠海马形态学改变。结果跳台实验中，与假手术组比较,模型组大鼠存在着明显的学习记忆障碍；与模型组比较,脑心通胶囊、喜得镇两治疗组大鼠学习记忆能力得到改善，且两组相比无显著性差异。光镜观察显示，假手术组大鼠海马CA1区锥体细胞排列紧密整齐，无明显神经元脱失；模型组大鼠海马C

2、A1区锥体细胞排列稀疏、紊乱，神经元脱失明显，可见胶质细胞增生，中、西药治疗组可明显减轻大鼠CA1区海马神经元脱失现象，使锥体细胞形态较正常，排列较整齐，接近假手术组。结论脑心通胶囊对血管性痴呆模型大鼠有治疗作用。关键词：脑心通胶囊;大脑中动脉闭塞;血管性痴呆;习记忆；海马TherapeuticEffectsofNaoxintongCapsuleonRatsModelofVascularDementiaAbstract:ObjectiveTostudytheeffectsofNaoxintongcapsuleonbehaviorandhippocampalhistomorph

3、ologyinvasculardementiarats.MethodsThevasculardementiamodelwasestablishedbyocclusionofthemiddlecerebralarteryinrats・Thefacultyoflearningandmemorywereevaluatedbystepdownavoidancetest・ThebraintissueswereremovedtobemadefrozensectionsandstainedwithHE,andthenthehippocampalhistomorphologywasobser

4、vedandanalyzedbylightmicrocope・ResultsTheresultsofstep-downavoidancetestshowedthatcomparedwithsham-operatedgroup,modelgrouppresentedsignificantlearningandmemorydisorder;comparedwithmodelgroup,NaoxintongcapsulegroupandHyderginegroupexhib辻edimprovementoflearningandmemorydisability.Meanwhile,t

5、hereexistednoobviousdifferencebetweenthesetwotherapygroups.Underthelightmicroscope,hippocampalCAIfieldofratsinthesham-operatedgroupexhibitedcloselyandneatlyspacedpyramidalcells,andinsignificantneuronloss;inthehippocampalCAIfieldofratsinthemodelgroupsparseanddisturbedpyramidalcells,noticeabl

6、eneuronlossandneuroglialcellproliferationcouldbeseen.NeuronlossreducedsignificantlyinthehippocampalCAIpyramidalcellfieldofthetwotherapygroupandcellmorphologypresentedrelativenormal.ConclusionNaoxintongcapsulehastherapeuticeffectonratmodelofvasculardementia・Keywords:Naoxintongcapsule;Middlec

7、erebralarteryocclusion;Vasculardementia;Learningandmemory;Hippocampus血管性痴呆(VascularDementia,VD)是因脑血管疾病所致的智能及认知能障碍临床综合征。随着社会人口老龄化和脑卒中治疗存活率的提高，VD的发病率愈来愈高。脑心通胶囊临床治疗缺血性脑卒中效果肯定，亦有学者将其扩展应用于VD的治疗[1],发现脑心通胶囊对VD的智能及其中医主症均有改善作用。本实验旨在通过观察脑心通胶囊对拟血管性痴呆模型大鼠行为学及脑组织形态学的