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1、IntramuscularAdministrationofZincMetallothioneintoPreslaughterStressedPigsImprovesAnti-oxidativeStatusandPorkQuality宰前肌肉注射锌金属硫蛋白提高猪抗氧化状态和猪肉品质L.L.Lil,Z.P.Houl,3,Y.L.Yinl,2,*,Y.H.Liu4,D.X.Hou5,B.Zhang4,GY.Wul,6,S.W.Kim7M.Z.Fan8,C.B.Yangl,X.F.Kongl,Z.R.Tangl,3,H.Z.Pcng4,D.Dcngl
2、,Z.Y.Dcng2M.Y.Xie2,H.Xiong2,P.KanglandS.X.Wangl1LaboratoryofAnimalNutritionandHealthandKeyLaboratoryofsubtropicalAgro-ecologyInstituteofSubtropicalAgriculture,TheChineseAcademyofSciences,Changsha,Hunan410125,P.R.ChinaABSTRACT:Thisstudywasconductedtodeterminetheeffectsofexoge
3、nouszinc-metallothionein(Zn-MT)onanti-oxidativefunctionandporkquality.Afterfeedingacorn-soybeanmeal-baseddietfortwoweeks,48pigs(DurocXLandraceXChineseBlackPig)wereassignedrandomlytofourgroups.PigsinGroup1weremaintainedundernon-stressconditions,whereaspigsinGroups2,3and4werea
4、ggressivelyhandledfor25mintoproducestress.PigsinGroups1,2,3,and4receivedintramuscularadministrationofsaline(controlgroup;CON),0(negativecontrolgroup;NCON),0.8(lowdosegroup;LOW),and1.6(highdosegroup;HIGH)mgrabbitliverZn-MTperkgbodyweight,respectively.Pigswereslaughteredat3and
5、6hpost-injection.Zn-MTtreatmentincreased(p<0.05)theactivitiesofsuperoxidedismutase(SOD)andglutathione-peroxidase(GSH-PX)whiledecreasingtheconcentrationofmalondialdehyde(MDA)inliver.Theseresponsesweregreater(p<0.05)at6hthanat3hpostZn-MTinjection.Zn-MTtreatmentincreased(p<0.05
6、)hepaticSODmRNAlevelsinatimeanddose-dependentmanneranddecreased(p<0.05)serumglutamate-pyruvatetransaminaseandlactatedehydrogenaseactivities(indicatorsoftissueintegrity).Zn-MTadministrationdecreased(p<0.05)lactateconcentrationandincreased(p<0.05)pHandwater-holdingcapacityinth
7、elongissimusthorasismeat.Collectively,ourresultsindicatethatintramuscularadministrationofZn-MTtopre-slaughterstressedpigsimprovedtissueanti-oxidativeabilityandmeatquality.KeyWords:Stress,Metallothionein,Anti-oxidativeEnzyme,SuperoxideDismutaseGeneExpression,PorkMeatQuality)摘
8、耍:这项研究是确定外源性锌-金属硫蛋口(Zn-MT)的抗氧化作用功能和对猪肉品质的影响。将48头猪(长白杜洛克屮国的黑猪)随机分为四组,并以玉、豆粕为