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1、RANKL和IL-17A在中耳胆脂瘤中的表达及相关性研究田斐李青峰冀永进马敏山西医科大学第二医院耳鼻咽喉头颈外科(太原030001)【摘要】目的观察破骨细胞分化因T(RANKL)和白介素-17A(1L-17A)在中耳胆脂瘤组织中的表达，探讨二者在中耳胆脂瘤骨质破坏机制中的作用。方法采用免疫组织化学两步法和计算机图像定量分析法，检测RAN・KL、IL-17A在25例中耳胆脂瘤上皮、16例胆脂瘤周潮肉芽组织及15例正常外耳道皮肤中的表达，同时观察二者的表达与临床听小骨骨质破坏程度的相互关系。结果(1)RANKL、IL-1
2、7A在胆脂瘤上皮及胆脂瘤周围肉芽组织中表达增高，分别与正常对照组比较，差异均有统计学意义(«fusxL=9.864.5.630,均戸<0.05；仏加=13.905、9.011,均P<0.05),且二者的表达与骨质破坏程度相关。(2)在胆脂瘤上皮及胆脂瘤周围肉芽组织中，RANKL与IL-17A的表达呈正相关(riff=0.692,“芽二0.538,Pv0.05)。结论RANKL及IL-17A在中耳胆脂瘤组织中呈高表达，与骨质破坏程度成正比，表明其与胆脂瘤骨质破坏机制密切相关；RANKL与IL-17A表达有相关性，提示二
3、者之间可能存在相互作用。【关键词］胆脂瘤，中耳；破骨细胞分化因子；白介素-I7A【中图分类号】R764.22,R349.39【文献标识码】A【文章编号】1672-2922(2010)01-090-05ExpressionofRANKLandIL-17AandtheircorrelationinmiddleearcholesteatomaTIANFei,LIQing-feng,JIYong-jin,MAMinDepartmentofOtorhinolaryngology-HeadandNeckSurgetheSecon
4、dHospitalofshanximedicalUniversity^Taiyuan,030001,China[Abstract]ObjectiveTostudyexpressionofRANKL(ReceptorActivatorofNuclearFactor-kappaBLigand)andIL-I7A(Interleukin-17A)inHumanMiddleEarCholestealornaandtheirmlesinbonedestruction.MethodsImmunohistochemicalmeth
5、odsandcomputer-aidedquantitativeimageanalysiswereusedtoexaminetheexpressionofRANKLandIL-17Ain25middleearcholesteatomaspecimens,16granulationtissuesurroundingthecholesteatomaand15specimensofnomiulexternalearcanalftkin.Hiecorrelationbetweenexpressionofthetwoindex
6、esandtheerosionofossicleswerealsoexamined.Results(I)IncreasedrexpressionofthecytokineRANKLandIL-17Awerefoundinthecholesteatomaepitheliumandsurn)un(linggranulationtissue^ascomparedwithnonnalexternalmeatalskin(/rank)=9.864,5.630,P<0.05;fn-i7A=13.905,9.011,P<0.05)
7、.Inaddition,expressionoftheIwoindexeswerecorrelatedtotheextentofossicledestruction.(2)Incholesteatomaepitheliumandgranulationtissue,expressionofRANKLwasdirectlycorrelatedwiththatofIL-17A(P<0.05).ConclusionRANKLandIL-I7Aareoverexpressedinmiddleearcholesteatomaan
8、dtheyareassociatedwithossicledestruction,indicatingthatRANKLandIL-17Aareresponsibleforbonedestructionincholesteatoma;RANKI,andIL-J7Ahaveacorrelationintheirexpression^suggest
