资源描述：
《rankl和opg在胆脂瘤型中耳炎中的表达及其意义》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、RANKL和OPG在胆脂瘤型中耳炎中的表达及其意义作者：丁寿玲作者单位：山东大学齐鲁医院耳鼻咽喉-头颈外科,济南250012【摘要】检测破骨细胞分化因子(nuclearfactorkappaBligand,RANKL)和护骨素(osteoprotegerin,OPG)在胆脂瘤型中耳炎组织中的表达水平，并探讨RANKL和OPG在胆脂瘤型中耳炎骨质破坏机制中的作用。方法应用逆转录聚合酶链反应(RTPCR)法和免疫组化SP法检测RANKL和OPG在35例中耳胆脂瘤(中耳炎组)和17例正常外耳道皮肤(对照组)中的表达。结果RTP
2、CR检测示胆脂瘤型中耳炎组与正常对照组比较,RANKL的mRNA表达水平升高(t=4.315;P<0.05),OPG的mRNA表达水平无明显变化(=0.578,P>0.05),RANKL与OPG的mRNA比值升高，差异均有统计学意义(t=6.127;P<0.05);免疫组化SP法检测显示RANKL在胆脂瘤组织中表达增高，主要在胆脂瘤周围浸润淋巴细胞胞浆屮表达，与对照组的表达比较差异有统计学意义(t=7.758,P<0.05);OPG在胆脂瘤组织中表达无明显变化，与对照组比较差异无统计学意义(t=0.623,P>0.05)
3、oOPG/RANKL在胆脂瘤组织中较对照组明显降低(t=8.183,Pv0.05)。结论RANKL在中耳胆脂瘤周围组织中表达增高，OPG的表达降低,与骨质破坏吸收关系密切;OPG/RANKL的降低可能是屮耳胆脂瘤骨质破坏的一个危险因素。DINGShouling,XIAMing,ZHANGHanbing,ZHANGJuhong,YANGYing,LIUFang,XUAnting(DepartmentofOtorhinolamygology&HeadandNeckSurgery,QiluHospitalofShandongU
4、niversity,Jinan250012,China)Abstract:ObjectiveToinvestigatetheexpressionofnuclearfactorkappaBligand(RANKL)andosteoprotegerin(OPG)inhumancholesteatomatissues.MethodThirtycholesteatomasamplesandtwentynormalauditorycanalskinspecimenswereenrolledinthisstudy,andexpres
5、sionsofRANKLandOPGweredeterminedbyreversetranscriptasepolymerasechainreaction(RTPCR)andimmunohistochemistry.ResultsThemRNAlevelofRANKL,butnotOPGwassignificantlyincreasedinthecholesteatomagroupcomparedwiththecontrolgroup(t=4.315,P<0.05;t=0.578,P>0.05).Meanwhile,th
6、eratioofOPG/RANKLwasincreased(t=6.127;P<0.05).ExpressionofthecytokineRANKLwasincreasedininfiltratedlymphocytesincholesteatomatissuescomparedwithnormalexternalmeatalskins(t=7.758,P<0.05),however,expressionofOPGwasnotsignificantlychangedincholesteatomatissuescompar
7、edwithnormalexternalmeatalskins(t=0.623;P>0.05).TheratioofOPG/RANKLwasdecreasedincholesteatomatissuescomparedwithnormalexternalmeatalskins(t=8.183;P<0.05).ConclusionThisstudyrevealsthattheexpressionlevelofRANKLismarkedlyincreasedintheperimatrixofcholesteatomaandi
8、scloselyrelatedtoboneresorptioninducedbycholesteatoma.ThedecreasedOPG/RANKLisaprobableriskfactorofboneresorptioninducedbycholesteatoma.Keywords:Cholesteatoma;N
