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ID:42624189
大小:40.23 KB
页数:10页
时间:2019-09-19
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1、EGCGregulatescellgrowth,cellcycleandphosphorylatednuclearfactor-KbinhumandermalfibroblastsEGCG对人体皮肤成纤维细胞的生长、细胞周期和磷酸化的核因子的影响Aim:toinvestigatetheeffectsofEGCG,themainpolyphenolingreentea,oncellgrowth,cellcycleandphosphorylatednuclearfactor-kBexpressioninneonatalhumandermalfibroblast.Methods:Thepro
2、liferation(增殖)andcell-cycleofnHDFsweredeterminedusingWST-8cellgrowthassay(测定)andflowcytometry(流体细胞仪),respectively(分别地).Theapoptosis(细胞凋亡)wasexaminedusingDNAladderandAnnexinV-FITCassays.TheexpressionlevelsofpNF-κBandcellcycle-relatedgenesandproteinsinnHDFsweremeasuredusingcDNAmicroarray(芯片)analys
3、esandWesternblot.Thecellularuptake(摄取)ofEGCGwasexaminedusingfluorescence(FITC)-labeledEGCG(FITC-EGCG)incombinationwithconfocalmicroscopy(多聚焦显微镜).Results:TheeffectofEGCGonthegrowthofnHDFsdependedontheconcentration(浓度)tested.Atalowconcentration(200μmol/L),EGCGresultedinaslightdecreaseintheproporti
4、on(比例)ofcellsintheSandG2/Mphasesofcellcyclewithaconcomitant(附随的)increaseintheproportionofcellsinG0/G1phase.Atthehigherdoses(剂量)(400and800μmol/L),apoptosis(细胞凋亡)wasinduced.Theregulation(调节)ofEGCGontheexpressionofpNF-κBwasalsoconcentration-dependent(浓度依赖性),whereas(但是)itdidnotaffecttheunphosphoryla
5、ted(磷酸化)NF-κBexpression.cDNAmicroarray(芯片)analysisshowedthatcellcycle-relatedgenesweredown-regulatedbyEGCG(200μmol/L).Theexpressionofcyclins(细胞周期蛋白)A/Bandcyclin-dependentkinase(致活酶)1wasreversibly(可逆地)regulatedbyEGCG(200μmol/L).FITC-EGCGwasfoundtobeinternalized(内化)intothecytoplasm(细胞质)andtransloc
6、ated(易位)intothenucleusofnHDFs.Conclusion:EGCG,throughuptake(摄取)intocytoplasm(细胞质),reversibly(可逆地)regulatedthecellgrowthandexpressionofcellcycle-relatedproteinsandgenesinnormalfibroblasts.IntroductionFoodbioactives(生物活性物质)withstrongantioxidant(抗氧化剂)properties(性能)havebeenshowntobecontainedingreent
7、ea,grapes,andturmericandhaveshowncancerchemopreventive(化学预防)andchemotherapeutic(化学疗法)effectsinmanyanimaltumor(肿瘤)bioassays(生物测定),cellculturesystems,andepidemiological(流行病学的)studies[1–3].Thesebiologicalactivitiesofgreenteapol
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