VEGF在非小细胞肺癌中表达与血管生成关系及临床意义

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1、-296-免疫学杂志第18卷第4期2002年7月IMMUNOLOGICALJOURNALVol18No4July2002[文章编号]1000-8861(2002)04-0296-03VEGF在非小细胞肺癌中表达与血管生成关系及临床意义温剑虎，朱冰(重庆医科大学附属第一医院胸外科，重庆400016)［播要］目的采用免疫组化方法观察血管内皮生长因子(VEGF)及血管内皮细胞膜抗原CD34在非小细胞肺癌(NSCLC)组织、肺炎性假瘤及正常肺组织中的表达状况。方法用抗VEGF多克隆抗体及CD34单克隆抗体作免疫组化染色，免疫标记物阳性细胞和癌组织中微血管密

2、度(MVD)计数。结果81例NSCLC组织上VEGF表达的总阳性率为72.84%,VEGF在肺癌细胞中主要分布于胞浆、胞膜，少量胞外基质也有阳性表达。鳞形细胞癌阳性细胞呈弥散或局灶分布，腺癌则呈腺泡状分布。VEB表达强度同非小细胞肺癌的MVD值紧密相关，随VEGF表达强度增高，MVD值亦显著增高(P<0.001)。结论VEGF的表达和MVD与NSCLC的发生、发展、转移关系密切，可作为NSCLC预后标志。［关■词］免疫组化;非小细胞肺癌;血管内皮生长因子;微血管密度［中图分类号］R734.2［文献标识码］AClinicalsignificanceo

3、fVEGFexpressionanditscorrelationwithangio^nesisinnon-small-cellranomaoflungWENJian・hu,ZHUBing(DepartmentofThoracicSurgery>FirstAffiliatedHospital,ChongqingUniversityofMedicalSciencestChongqing400016,China)[Abstract]ObjectivesToinvestigatetheinvolvementofVEGFintheoccurrenceandd

4、evelopmentofnon-small-cellcarcinomaoflungbyexaminationoftheexpressionofVEGFandCD34betweenNSCLC9inflammatorypseudotumoroflungandnormallungtissuewithimmunohistochemicalstaining,tostudytherelationshipbetweenVEGFexpressionandangiogenesisinNSCLC.MethodsImmunohistochemicalstaining

5、waspeHormedbyanti-VEGFpolyclonalantibodyandanti-CD34monoclonalantibodies,andthedegreeofantibodyreactivitywithindividualtissuesectionswasconsideredtobepositiveifunequivocalbrown-yellowstainingofmembraneorcytoplasmwasseenintumorcells.ResultsThetotalpositiverateof81patientsis72.8

6、4%.VEGFwasmainlydistributedincytoplasmandmembraneoftumorcell>sometumorsdemonstratedpositivestaininginthebasallaminaorextracellularstroma9sodidsomenormaltissue.PositivecellshoweddiffuseorlocaldifiusioninSQCCandsurroundedaroundadeno-alveolainADC・Itwasfoundthattherewasacloserela

7、tionshipbetweenMVDandVEGFexpressioninNSC1QandMVDincreasedwiththehigherexpressionofVEGF(P<0.001).ConclusionExpressionofVEGFiscloselyrelatedtotheoccurrenceanddevelopmentofNSCIX2andplaysanimportantroleinneoontogenesis9itmayactasadiagnosticsmarkerforclinicalbiologicalcharacterist

8、icinNSCLC.[Keywords]immunohistochemistry;nonsmallcelllungcarc