Lignite pre-treatment and its effect on bio-stimulative properties of respective

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1、SoilBiology&Biochemistry41(2009)1894–1901ContentslistsavailableatScienceDirectSoilBiology&Biochemistryjournalhomepage:www.elsevier.com/locate/soilbioLignitepre-treatmentanditseffectonbio-stimulativepropertiesofrespectivelignitehumicacidsabcaa,*ZojaVlcˇkova´,LaurentGrasset,BarboraAntosˇova´,

2、MiloslavPekarˇ,Jirˇı´Kucˇerı´kaBrnoUniversityofTechnology,FacultyofChemistry,Purkynˇova118,61200Brno,CzechRepublicbUniversite´depoitiers,SRSN,UMRCNRS6514,40Av.duRecteur-Pineau,86022Poitiers,FrancecResearchInstituteofInorganicChemistry,Revolucˇnı´1521/84,40001U´stı´nadLabem,CzechRepublicarti

3、cleinfoabstractArticlehistory:HumicacidsoriginatingfromSouth-MoravianligniteweresubjectedtoacomparativestudywiththeaimtoReceived9March2009assessthealterationoftheirphysico-chemicalpropertiesaftervariouslignitepre-treatments.PhysicalReceivedinrevisedformmodificationwasachievedwithtwoorganicac

4、ids,suchasaceticacidandcitricacidandchemicalmodi-9June2009ficationbynitricacidandhydrogeneperoxideinvariousconcentrations.Elementalanalysis,solid-stateAccepted25June2009NMR,GC–MSanalysisofpolyolsandsizeexclusionchromatographywerecarriedoutforchemical–physicalAvailableonline14July2009characte

5、rizationofobtainedhumicacids.Theirbiologicaleffect,informofpotassiumandammoniumhumates,wastestedonmaize(Zeamays)seedlings.Inthesetests,potassiumhumatesachievedfarbetterKeywords:overallresultsthanammoniumhumates.Resultswereinter-correlatedinordertoappraisetheinfluenceofLignitehumicacidsOxidat

6、ionhumicacidsphysicalandchemicalpropertiesonbiologicalactivity.Surprisingly,fractionswiththelowestModificationmolecularsize(0–35kDa)showednocorrelationwithbioactivityresults(Pearsoncoefficientfrom0.05toBiologicalactivity0.4).Onthecontrary,middle-sizedfractions(35–175kDa)showedhighlysignifican

7、tpositivecorrelationAceticacid(Pearsoncoefficientupto0.92)andthehighestmolecular-size-fractions(275–350kDa)showednegativeCitricacidcorrelation(Pearsoncoefficientupto0.75).Thesefindingswereidenticalforbothpotassiumandammoniumhumates.Noconnectionwasfoundbetw