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1、TheJapaneseSocietyofDevelopmentalBiologistsDevelop.GrowthDiffer.(2013)55,523–549doi:10.1111/dgd.12054ReviewArticleImageprocessingandrecognitionforbiologicalimagesSeiichiUchida*DepartmentofAdvancedInformationTechnology,KyushuUniversity,744Motooka,Nishi,Fukuoka,819-0395,Ja
2、panThispaperreviewsimageprocessingandpatternrecognitiontechniques,whichwillbeusefultoanalyzebioimages.Althoughthispaperdoesnotprovidetheirtechnicaldetails,itwillbepossibletograsptheirmaintasksandtypicaltoolstohandlethetasks.Imageprocessingisalargeresearchareatoimprovethe
3、visibilityofaninputimageandacquiresomevaluableinformationfromit.Asthemaintasksofimagepro-cessing,thispaperintroducesgray-leveltransformation,binarization,imagefiltering,imagesegmentation,visualobjecttracking,opticalflowandimageregistration.Imagepatternrecognitionisthetechn
4、iquetoclas-sifyaninputimageintooneofthepredefinedclassesandalsohasalargeresearcharea.Thispaperover-viewsitstwomainmodules,thatis,featureextractionmoduleandclassificationmodule.Throughoutthepaper,itwillbeemphasizedthatbioimageisaverydifficulttargetforevenstate-of-the-artimag
5、eprocessingandpatternrecognitiontechniquesduetonoises,deformations,etc.Thispaperisexpectedtobeonetuto-rialguidetobridgebiologyandimageprocessingresearchersfortheirfurthercollaborationtotacklesuchadifficulttarget.Keywords:bioimageinformatics,imagepatternrecognition,imagepr
6、ocessing.thermore,weneedtobecarefulthattheanalysisIntroductionresultbythemanualinspectionmaybebiasedbysub-Developmentofimagingtechniques,especially,micro-jectiveobservation.Thatis,theanalysisresultwillscopicimagingtechniques,hasmadeitpossibletodependlargelyonpersonalskil
7、l,decision,andprefer-visualizevariousbiologicalphenomenaforbiologists.ence.Imagingresultsareprovidedasstill-imagesorvideos.Ontheotherhand,researchon“bioimage-informat-Inmoreelaboratecases,3Dvolumetricimagesandics”isbecomingactive.Thepurposeofbioimage-4Dvolumetricvideosar
8、eprovided.Behavioranddistri-informaticsistoanalyzebioimagesautomaticallyorbutionofmoleculesarealsovisua