资源描述：
《MicroRNA signature in response to nutrient restriction and re-feeding响应营养限制和再投喂的microRNA特征 草鱼快速骨骼肌的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ZoologicalResearch35(5):404−410DOI:10.13918/j.issn.2095-8137.2014.5.404MicroRNAsignatureinresponsetonutrientrestrictionandre-feedinginfastskeletalmuscleofgrasscarp(Ctenopharyngodonidella)1,22211,*2,*XinZHU,Wu-YingCHU,PingWU,TanYI,TaoCHEN,Jian-SheZHANG1.CollegeofVete
2、rinaryMedicine,HunanAgricultureUniversity,ChangshaHunan410128,China2.DepartmentofBioengineeringandEnvironmentalScience,ChangshaUniversity,ChangshaHunan410003,ChinaAbstract:Thegrasscarp(Ctenopharyngodonidella)isoneofthemostimportantcultivatedfishspeciesinChina.Mounti
3、ngevidencessuggeststhatmicroRNAs(miRNAs)maybekeyregulatorsofskeletalmuscleamongthegrasscarp,buttheknowledgeoftheidentityofmyogenicmiRNAsandroleofmiRNAsduringskeletalmuscleanabolicstateremainslimited.Inthepresentstudy,wechoose8miRNAspreviouslyreportedtoactasmusclegro
4、wth-relatedmiRNAsforfasting-refeedingresearch.Weinvestigatedpostprandialchangesintheexpressionof8miRNAsfollowingasinglesatiatingmealingrasscarpjuvenileswhohadbeenfastingforoneweekandfoundthat7miRNAsweresharplyup-regulatedwithin1or3hafterrefeeding,suggestingthattheym
5、aybepromisingcandidatemiRNAsinvolvedinafast-responsesignalingsystemthatregulatesfishskeletalmusclegrowth.Keywords:MicroRNA;Grasscarp;Fasting;Refeeding;SkeletalmuscleThegrasscarp(Ctenopharyngodonidella)isoneofotingmRNAdegradationthroughbasepairingtothe3'themostimport
6、antcultivatedspeciesinChinesefreshw-untranslatedregion(UTR)oftargetmRNAs(Xieetal,1ateraquaculture.Generally,fastskeletalmusclecompri-2005;Liu,2008;Zhang&Wen,2010).Furthermore,sesthelargesttissueofthefishbody(Zhangetal,2009),miRNAsregulatetheexpressionoftranscription
7、factorsandformsthemainediblepartofthisspecies.Giventheandsignalingmediatorscriticaltobothcardiacandcomplicateddevelopmentprocessaswellastheimpor-skeletalmuscledevelopmentandfunction(Callis&tanceofthesetissues,gainingaclearerunderstandingofWang,2008;vanRooijetal,2008
8、).StudiesusingthethemechanismunderlyingmuscledevelopmentmaymousemyogenicC2C12celllinedemonstratedmiR-1providekeydataforbothdevelopmentalbi
