Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ

《Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、JournalofPeptideScienceJ.PeptideSci.5:289±297(1999)PreparationandCharacterizationofNovelBioactivePeptidesResponsibleforAngiotensinI-ConvertingEnzymeInhibitionfromWheatGermTOSHIROMATSUI*,CHUN-HUILIandYUTAKAOSAJIMADepartmentofFoodScienceandTechnology,KyushuUnive

2、rsity,Fukuoka812-8581,JapanReceived2October1998Accepted8December1998Abstract:Reportedisthepreparationofwheatgerm(WG)hydrolyzatewithpotentangiotensinI-convertingenzyme(ACE)inhibitoryactivity,andthecharacterizationofpeptidesresponsibleforACEinhibition.Successful

3、hydrolyzatewiththemostpotentACEinhibitoryactivitywasobtainedby0.5wt.%±8hBacilluslicheniformisalkalineproteasehydrolysisafter3.0wt.%±3ha-amylasetreatmentofdefattedWG(IC50;0.37mgproteinml1).TheactivityofWGhydrolyzatewasmarkedlyincreasedbyODSandsubsequentAG50Wpu

4、rifications(IC;0.018mgproteinml1).Asaresultofisolationsbyhighperformanceliquidchro-50matographies,16peptideswiththeIC50valueoflessthan20mM,composedof2±7aminoacidresidueswereidentifiedfromtheWGhydrolyzate.Judgingfromthehighcontent(260mgin100gofAG50Wfraction)an

5、dpowerfulACEinhibitoryactivity(IC50;0.48mM),Ile-Val-Tyrwasidentifiedasamaincontribu-tortotheACEinhibitionofthehydrolyzate.Copyright©1999EuropeanPeptideSocietyandJohnWiley&Sons,Ltd.Keywords:ACEinhibitorypeptide;hypertension;wheatgermhydrolyzate;alkalineprotease

6、INTRODUCTIONtheseactiveANGsorretardingthecatalyticactionofangiotensinI-convertingenzyme(ACE,ECAngiotensins(ANGs),inparticularANGII,III,and3.4.15.1)[1].Asapointofthisview,syntheticACE(1±7)areknowntoplayimportantphysiologicalinhibitors,suchascaptopril(IC50;21nM)

7、androlesasapressorsubstanceinthecirculatoryand:enalapril(IC50;3nM)havebeendevelopedandsub-orlocalizedrenin±angiotensinsystems.Therefore,jectedtoclinicaluse[2].pharmatherapeuticexaminationstosuppresstheThestudyofthepreparationandidentificationofpromotionofblood

8、pressureinhypertensiveshavenaturalACEinhibitorsderivedfromfoodstuffshasbeenattemptedbyinhibitingtheproductionofbecomeanactiveareaofresearchtomaintainourhomeostasisbytakingfood[3±5]