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1、PurificationandCharacterizationofAngiotensinI-ConvertingEnzymeInhibitorsfromSourMilkYASUNORINAKAMURA,1NAOYUKIYAMAMOTO,KUMISAKAI,AKIRAOKUBO,2SUNAOYAMAZAKI,2andTOSHIAKITAKANOResearch&DevelopmentCenterTheCalpisFoodIndustryCo.,Ltd.&-11-10Fuchinobe,SagamiharaKanagawa229,JapanandDe
2、partmentofAgriculturalChemistryFacultyofAgricultureTokyoUniversity,Bunkyo-kuTokyo113,JapanABSTRACTSaccharomycescerevisiae.Calpissourmilkhasseveralphysiologicaleffects,includingex•TheinhibitoryactivityofangiotensintendedlifespaninICRmiceandinhibitionofI-convertingenzymeinmilki
3、ncreasedchemicallyinducedtumorigenesisinF344ratsduringfermentationwiththeCalpissourmilkstartercontainingLactobacillushel•(1,17).BloodpressureincreasewasinhibitedveticusandSaccharomycescerevisiae.bylong-termfeedingofthesourmilktotheTwokindsofpeptidesinhibitorytoan•spontaneousl
4、yhypertensiveSHRstrainofratsgiotensinI-convertingenzymewerepuri•(K.Wada,personalcommunication).fiedfromthesourmilkbyusingfour•AngiotensinI-convertingenzyme(ACE)isstepHPLC.Theaminoacidsequencesofimportantinregulationofbloodpressurebe•theseinhibitorswereidentifiedasVal•causeitc
5、atalyzestheformationofthepotentPro-ProandDe-Pro-Pro.Theconcentra•vasopressorangiotensinIIfromangiotensinItionsofpeptidesproviding50%inhibi•(15).SomeinhibitorsofACEshowantihyper•tionofangiotensinI-convertingenzymetensiveeffects(14)andareusedaspharmaceu•were9and5pM,respectively
6、.Mostofticals.TheACEinhibitoryactivityoffoodstheinhibitoryactivityinsourmilkwashasbeenstudied(8,16),andsomeACEinhibi•attributedtothesetwopeptides.torypeptideswereproducedbytheenzymatic(Keywords:angiotensinI-convertinghydrolysisofcasein(11,12).YamamotoetaI.enzyme,inhibitor,sou
7、rmilk,purifica•(18,19)reportedthatcasein,hydrolyzedbytion)proteinasefromLhelveticusCP790thatwasisolatedfromtheCalpissourmilkstarter,Abbreviationkey:ACE=angiotensin1•showedACEinhibitoryactivityandalsoanti•convertingenzyme,Hip-His-Leu=Hippuryl•hypertensiveactivityinSHRrats.More
8、over,L-histidyl-L-Ieucine,ICso=theconcentrationYamamotoetaI.(18)repo
