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1、Availableonlineatwww.sciencedirect.comApproachesfornovelenzymediscoveryfrommarineenvironments1,21,21,2HyunSookLee,KaeKyoungKwon,SungGyunKang,1,21,21,2Sun-ShinCha,Sang-JinKimandJung-HyunLeeTheenormouspoolofbiodiversityinmarineecosystemsisandate,manyresearchinstitute
2、sandcompanieshaveestab-excellentnaturalreservoirforacquiringaninventoryoflishedcollectionsoforganismsfromavarietyof‘common’enzymeswithpotentialforbiotechnologicalapplications.environments(e.g.soilorseawater)andextremeenviron-Moreover,theopportunityforsustainableres
3、ourcements(e.g.hotsprings,Antarcticice,alkalinelakes,etc.),managementhasbeengreatlyenhancedbyrecentadvancesinyieldingavarietyofenzymesthatcatalyzereactionsculturingmethodsforrecalcitrantmicrobes.Inthisreview,weundernormalorextremeconditions.Therearealsowillfocuspri
4、marilyonsuccessfulexamplesinculturingmarinehighlychangeable,thoughnotparticularlyextreme,microbesandprovideanoverviewofworkexaminingtheenvironments,whicharesubjecttolargefluctuationsinbiotechnologicalpotentialofthemarinereservoir,mainlyparameterssuchastemperature,pH
5、orsalinity.Thesethroughgenomicstrategies,suchasactivity-basedfunctionalbecomepopulatedbycommunitiesoforganismswithscreeningofgenomicandmetagenomiclibrariesandintrinsicgenomicheterogeneity,whichmaybeasourcehomology-drivenscreeningofenormousamountsofsequenceofenzymes
6、thatshowahigherlevelofstabilityanddata.activityoverawiderrangeofconditions.Addresses1OrganismsthatcanbeculturedencompassonlyatinyKoreaOceanResearchandDevelopmentInstitute,Ansan,P.O.Box29,Seoul425-600,SouthKoreafractionoftheexistinggenomicdiversityinanygiven2Departm
7、entofMarineBiotechnology,UniversityofScienceandenvironment,thus,screeningonlythesewouldlimittheTechnology,Daejeon305-333,SouthKoreapotentialspectrumofnewenzymesfoundforthebiopro-Correspondingauthor:Lee,Jung-Hyun(jlee@kordi.re.kr)cessindustry.Nevertheless,thefacttha
8、talargenumberofproteinsequencesdeducedfromthegenomesoftheseculturedorganismsstillhavenoassignedfunction,givesaCurrentOpinioninBiotechnology2010,2
