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1、32ndAnnualInternationalConferenceoftheIEEEEMBSBuenosAires,Argentina,August31-September4,2010Post-BioprintingProcessingMethodstoImproveCellViabilityandPatternFidelityinHeterogeneousTissueTestSystemsMatthewE.Pepper,Member,IEEE,CherylA.P.Cass,JustinP.Matt
2、imore,Member,IEEETimothyBurg,SeniorMember,IEEE,BrianW.Booth,KarenJ.L.Burg,SeniorMember,IEEE,andRichardE.Groff,Member,IEEEWhiledevelopingthistissuetestsystem,severalAbstract—Bioprintedtissuetestsystemsshowpromiseasapowerfultoolforstudyingcell-cellintera
3、ctioninchallengeswereencounteredinprocessingsamplesafterheterogeneous,tissue-likeco-culture.Severalchallengeswerebioprintingapattern.Iftheapplicationofmediumisencounteredwhileattemptingtoconsistentlyfabricatesamplesdelayed,theviabilityofasampleisdimini
4、shed(e.g.Fig.withhighviabilityandpatternfidelity.Thispaperevaluates1b).Alternatively,ifmediumisapplieddirectlyafterfourmethodsforprocessingsamplesafterbioprintingbutpriorprinting,thepatternfidelityisdegradedsincethecellshavetoaddingmediaforincubation.T
5、hesemethods,composedofnothadsufficienttimetoattachtothesubstrate(e.g.Fig.1c)variouscombinationsofthreetechniquesmeanttopromotecellandcanbedisplacedfromtheprintedlocation.Basedonhydration,areevaluatedwithrespecttosampleviabilityandpatternpreservation.In
6、thebestperformingmethod,Hank’sobservation,thedecreasedviabilityisbelievedtobeduetoBalancedSaltSolutionwasappliedimmediatelyafterdehydrationofthecells.Thismotivatesasearchforfabricationandacollagenoverlayerwasappliedonehourtreatmentstokeepthecellshydrat
7、edwithoutdisturbingtheirthereafter.Thesuccessofthismethodhighlightstheabilityofposition.thecollagensubstratetoabsorbmoisture,whichpromotescellThispaperpresentsanevaluationofseveralpost-healthwithoutdisturbingthecell’sprintedlocation.Anbioprintingproces
8、singmethodsintermsofmaintainingaddendumtothemainstudyisaninvestigationofthelimitsofconsistentlyhighcellviabilityandpatternfidelity.EachanHP26printcartridgetodepositcellsatafasterrateforthemethodconsistsofasetoftreatmentsappliedatspecifi
