chapter16 Gene regulation in eukaryotes

《chapter16 Gene regulation in eukaryotes》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、Chapter16Chapter16GeneRegulationinEukaryotesGeneRegulationinEukaryotesCopyright©TheMcGraw-HillCompanies,Inc.Permissionrequiredtoreproduceordisplay18-1OutlineofChapter16OutlineofChapter16HowweusegeneticstostudygeneregulationHowweusegeneticstostudygeneregulationUsingmuta

2、tionstoidentifycis-actingelementsandtrans--actingproteinsHowgenesareregulatedattheinitiationofHowgenesareregulatedattheinitiationoftranscriptiontranscriptionThreepolymerasesrecognizethreeclassesofpromoters.Trans-actingproteinscontrolclassIIpromoters.Chromatinstructur

3、eaffectsgeneexpression.GenomicimprintingandDNAmethylationHowgenesareregulatedaftertranscriptionHowgenesareregulatedaftertranscriptionRNAsplicingMicro-RNAProteinmodificationandstabilityCopyright©TheMcGraw-HillCompanies,Inc.Permissionrequiredtoreproduceordisplay18-2Di

4、fferencesofgeneregulationineukaryotescomparedwithprokaryotesEukaryoticgenomescarryfarmoreDNA,challengingEukaryoticgenomescarryfarmoreDNA,challengingforproteinstolocatebindingsequences.forproteinstolocatebindingsequences.ChromatinstructuremakesDNAunavailabletoChromatins

5、tructuremakesDNAunavailabletotranscriptionmachinery.transcriptionmachinery.AdditionalRNAprocessingeventsoccur.AdditionalRNAprocessingeventsoccur.Transcriptionandtranslationarespatiallyseparated.Transcriptionandtranslationarespatiallyseparated.Copyright©TheMcGraw-HillCo

6、mpanies,Inc.Permissionrequiredtoreproduceordisplay18-3GeneexpressionineukaryotesFig.16.1Copyright©TheMcGraw-HillCompanies,Inc.Permissionrequiredtoreproduceordisplay18-4Copyright©TheMcGraw-HillCompanies,Inc.Permissionrequiredtoreproduceordisplay18-5Ineukaryotes,threeRNApo

7、lymerasesIneukaryotes,threeRNApolymerasestranscribedifferentsetsofgenestranscribedifferentsetsofgenesRNApolymeraseIRNApolymeraseItranscribestranscribesrRNArRNA..rRNAsrRNAsaremadeoftandemrepeatsononeormorechromosomes.RNApolymeraseItranscribesoneprimaryrRNArRNAtranscrip

8、twhichisfurtherprocessedto28S,5.8S,and18SrRNA.Copyright©TheMcGraw-HillCompanies,Inc.Permissionrequiredt